Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA.
Analyst. 2017 Sep 25;142(19):3605-3612. doi: 10.1039/c7an00794a.
This paper describes the use of 3D microtissues as an intermediate model between the 2D cell culture and the animal model to assess radiation-induced cellular and DNA damage in the context of personalized radiation therapy. An agarose microwell array was used to generate 3D microtissues with controlled size and shape. The microtissues were exposed to X-ray radiation of various doses, and the radiation damage to cells was examined using a variety of techniques with different end points. Damage to cell membranes and reduction in metabolic activity were examined with the MTT assay and dye inclusion assay. DNA damage was tested with the micronucleus assay, γ-H2AX immunostaining, and HaloChip assay. 3D microtissues exposed to X-rays are smaller compared to unexposed ones in extended cultures, indicating that X-ray radiation can retard the growth of cells in 3D microtissues, where cells at the outer shells of microtissues can prevent further damage to those inside.
本文介绍了 3D 微组织在 2D 细胞培养和动物模型之间的应用，作为评估个性化放疗中辐射诱导的细胞和 DNA 损伤的中间模型。使用琼脂糖微井阵列生成具有可控大小和形状的 3D 微组织。用不同剂量的 X 射线照射微组织，并使用不同终点的各种技术检查细胞的辐射损伤。用 MTT 测定法和染料掺入法检测细胞膜损伤和代谢活性降低。用微核测定法、γ-H2AX 免疫染色法和 HaloChip 测定法检测 DNA 损伤。与未暴露于 X 射线的微组织相比，延长培养后的微组织体积较小，这表明 X 射线辐射可以减缓 3D 微组织中细胞的生长，其中微组织外壳层的细胞可以防止内部细胞受到进一步的损伤。
