a Department and Institute of Infectious Disease, Tongji Hospital, Tongji Medical College , Huazhong University of Science and Technology , Wuhan , Hubei , China.
b Department of Endocrinology Affiliated Liyuan Hospital, Tongji Medical College , Huazhong University of Science and Technology , Wuhan , Hubei , China.
Cancer Biol Ther. 2017 Sep 2;18(9):723-729. doi: 10.1080/15384047.2017.1360451. Epub 2017 Aug 16.
To investigate the expression of endothelin receptors in liver diseases and discuss its role in the process of liver cirrhosis and liver cancer.
We examined the expressions of ETAR, ETBR and α-SMA in tissue samples using western blotting analysis. Furthermore, immunofluorescence was used to locate ETAR expression in hepatic stellate cells (HSCs) and hepatic sinusoidal endothelial cells (HSECs), we calculated the percentage of positive cells and then analyzed its relation with clinical indexes.
According to the western blotting analysis, the expression of ETAR was high in hepatic hemangioma and liver cancer tissues and ETBR was highly expressed in cirrhosis tissues. The immunofluorescence results demonstrated that the expression of ETAR was elevated in hepatic hemangioma and liver cancer tissues. Moreover, ETAR expression was found in both HSCs and HSECs. Finally, the statistical analysis revealed that the number of positive ETAR cells was correlated with the clinical index platelets (PLT), alanine transaminase (ALT) and diameter of portal vein.
Endothelin receptors express differently in liver cirrhosis and liver cancer tissues and play a role in hepatic diseases by affecting HSCs and HSECs.
研究内皮素受体在肝脏疾病中的表达，探讨其在肝硬化和肝癌发生发展过程中的作用。
采用 Western blot 分析检测组织样本中 ETAR、ETBR 和α-SMA 的表达情况。免疫荧光定位 ETAR 在肝星状细胞（HSCs）和肝窦内皮细胞（HSECs）中的表达，计算阳性细胞的百分比，并分析其与临床指标的关系。
Western blot 分析显示，ETAR 在肝血管瘤和肝癌组织中高表达，ETBR 在肝硬化组织中高表达。免疫荧光结果显示，ETAR 在肝血管瘤和肝癌组织中表达上调。此外，ETAR 表达存在于 HSCs 和 HSECs 中。统计学分析显示，阳性 ETAR 细胞数量与血小板（PLT）、丙氨酸转氨酶（ALT）和门静脉直径等临床指标相关。
内皮素受体在肝硬化和肝癌组织中的表达不同，通过影响 HSCs 和 HSECs 在肝脏疾病中发挥作用。
