聚乙二醇化重组人血管内皮抑制素钠在大鼠椎板切除术后硬膜外纤维化抑制中的作用

The role of pegaptanib sodium in the suppression of epidural fibrosis in a postlaminectomy rat model.

作者信息

Sertbas I, Yilmaz A, Yildirim T, Karatay M, Celik H, Bayar M A

出版信息

Bratisl Lek Listy. 2017;118(2):118-122. doi: 10.4149/BLL_2017_024.

DOI:10.4149/BLL_2017_024

PMID:28814094

Abstract

OBJECTIVE

Spinal epidural fibrosis is a clinical condition that develops after laminectomy and can compress the spine. Many agents have been tried for the treatment, but none has entered clinical use at present. Pegaptanib sodium is an antiangiogenetic drug that prevents the development of new vessels and thus adhesion by inhibiting the effect of VEGF.

MATERIAL AND METHOD

20 Wistar rats were used in this study. The rats were divided into 2 different groups as the control and pegaptanib sodium group. Three levels of laminectomy were performed. Only laminectomy was performed in the control group. A cotton ball soaked with 3 mg/kg Pegaptanib sodium diluted 1: 10 with 0.9 % NaCl was topically applied to the dura in the surgical field for 5 minutes in the pegaptanib sodium group. The rats were sacrificed 3 weeks later and histopathologically examined. The epidural fibrosis was graded.

RESULTS

The epidural fibrosis grade in the pegaptanib sodium was significantly lower than in the control group c2 = 11,65; (p = 0.004)CONCLUSION: Pegaptanib sodium blocked the VEGF through its anti-VEGF effect and decreased spinal epidural fibrosis in rats that had undergone laminectomy (Tab. 2, Fig. 3, Ref. 53).

摘要

目的

脊柱硬膜外纤维化是一种在椎板切除术后出现的临床病症，可压迫脊柱。已经尝试了多种药物进行治疗，但目前尚无药物进入临床应用。培加他汀钠是一种抗血管生成药物，通过抑制血管内皮生长因子（VEGF）的作用来阻止新血管的形成，从而防止粘连。

材料与方法

本研究使用了20只Wistar大鼠。将大鼠分为对照组和培加他汀钠组两个不同的组。进行了三个节段的椎板切除术。对照组仅进行椎板切除术。在培加他汀钠组中，将用0.9%氯化钠按1:10稀释的3mg/kg培加他汀钠浸泡的棉球局部应用于手术区域的硬脑膜5分钟。3周后处死大鼠并进行组织病理学检查。对硬膜外纤维化进行分级。