共价结合的吡咯喹啉醌是人类胎盘赖氨酰氧化酶中的有机辅基。
Covalently bound pyrroloquinoline quinone is the organic prosthetic group in human placental lysyl oxidase.
作者信息
van der Meer R A, Duine J A
出版信息
Biochem J. 1986 Nov 1;239(3):789-91. doi: 10.1042/bj2390789.
Abstract
Treatment of purified human placental lysyl oxidase with 2,4-dinitrophenylhydrazine (DNPH) resulted in a large spectral change and inhibition of enzyme activity. Proteolytic degradation of the derivatized enzyme yielded only one single coloured product, which was spectrally and chromatographically identical with the C-5 hydrazone of PQQ (pyrroloquinoline quinone) and DNPH. Since this represents the first example of a PQQ-containing enzyme in man, possible implications of the finding are discussed.
摘要
用2,4 - 二硝基苯肼（DNPH）处理纯化的人胎盘赖氨酰氧化酶会导致光谱发生很大变化并抑制酶活性。对衍生化酶进行蛋白水解降解仅产生一种单一的有色产物，其在光谱和色谱上与PQQ（吡咯并喹啉醌）和DNPH的C - 5腙相同。由于这是人类中含PQQ酶的首个实例，因此讨论了该发现可能的影响。
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本文引用的文献
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