墨西哥儿科患者中枢神经系统肿瘤中微小RNA的表达
Expression of microRNAs in tumors of the central nervous system in pediatric patients in México.
作者信息
Eguía-Aguilar Pilar, Gutiérrez-Castillo Lisette, Pérezpeña-Díazconti Mario, García-Chéquer Jeanette, García-Quintana Jorge, Chico-Ponce de León Fernando, Gordillo-Domínguez Luis, Torres-García Samuel, Arenas-Huertero Francisco
机构信息
Departmento de Patología Clínica y Experimental, Hospital Infantil de México Federico Gómez, Ciudad de México, México.
Facultad de Ciencia y Tecnología, Universidad Simón Bolívar, Ciudad de México, México.
出版信息
Childs Nerv Syst. 2017 Dec;33(12):2117-2128. doi: 10.1007/s00381-017-3569-9. Epub 2017 Aug 16.
PURPOSE
MicroRNAs were identified as molecules that participate in gene regulation; alterations in their expression characterize central nervous system (CNS). Information in pediatrics is scarce, so the objective of this work was to determine and then compare the patterns of expression of microRNAs in astrocytomas, ependymomas, and medulloblastomas, as well as in non-neoplastic brain.
METHODS
Low-density arrays were utilized to evaluate 756 microRNAs in three samples of each type of tumor and non-neoplastic brain. The relative expression was calculated in order to identify the three microRNAs whose expression was modified notably. This was verified using RT-qPCR in more number of tumor samples.
RESULTS
The microRNAs selected for testing were miR-100-5p, miR-195-5p, and miR-770-5p. A higher expression of miR-100-5p was observed in the astrocytomas and ependymomas compared to the medulloblastomas: on average 3.8 times (p < 0.05). MiR-770-5p was expressed less in medulloblastomas compared to astrocytomas four times (p = 0.0162). MiR-195-5p had a low expression in medulloblastomas compared to non-neoplastic cerebellum (p = 0.049). In all three tumor types, expression of miR-770-5p was lower than in non-neoplastic brain (p < 0.001).
CONCLUSIONS
These microRNAs may represent potential markers in these tumors.
目的
微小RNA被鉴定为参与基因调控的分子；其表达改变是中枢神经系统（CNS）的特征。儿科方面的信息较少，因此本研究的目的是确定并比较星形细胞瘤、室管膜瘤和髓母细胞瘤以及非肿瘤性脑内微小RNA的表达模式。
方法
利用低密度阵列评估每种肿瘤类型和非肿瘤性脑的三个样本中的756种微小RNA。计算相对表达量以鉴定三种表达有显著改变的微小RNA。通过对更多肿瘤样本进行逆转录定量聚合酶链反应（RT-qPCR）来验证这一结果。
结果
选择用于检测的微小RNA为miR-100-5p、miR-195-5p和miR-770-5p。与髓母细胞瘤相比，在星形细胞瘤和室管膜瘤中观察到miR-100-5p表达更高：平均高3.8倍（p<0.05）。与星形细胞瘤相比，髓母细胞瘤中miR-770-5p的表达低四倍（p = 0.0162）。与非肿瘤性小脑相比，髓母细胞瘤中miR-195-5p表达较低（p = 0.049）。在所有三种肿瘤类型中，miR-770-5p的表达均低于非肿瘤性脑（p<0.001）。
结论
这些微小RNA可能是这些肿瘤的潜在标志物。
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