Wolin M S, Burke T M
Biochem Biophys Res Commun. 1987 Feb 27;143(1):20-5. doi: 10.1016/0006-291x(87)90623-1.
Guanylate cyclase activity in the soluble extract of bovine pulmonary arteries is activated by hydrogen peroxide generated by glucose oxidase only in the presence of catalase. This mechanism of guanylate cyclase activation is not blocked by scavengers for superoxide anion or hydroxyl radical, but is selectively inhibited by methylene blue, inactivation of catalase and ethanol. The time dependency of increases in guanylate cyclase activity in the presence of peroxides that are substrates for catalase are associated with the spectral detection of compound I, a species of catalase formed during the metabolism of peroxide. Thus, activation of soluble guanylate cyclase appears to be elicited by compound I of catalase or by a mediator generated by this species.
仅在过氧化氢酶存在的情况下，葡萄糖氧化酶产生的过氧化氢可激活牛肺动脉可溶性提取物中的鸟苷酸环化酶活性。这种鸟苷酸环化酶激活机制不受超氧阴离子或羟基自由基清除剂的阻断，但可被亚甲蓝、过氧化氢酶失活和乙醇选择性抑制。在过氧化氢酶底物过氧化物存在下，鸟苷酸环化酶活性增加的时间依赖性与化合物I的光谱检测相关，化合物I是过氧化物代谢过程中形成的一种过氧化氢酶。因此，可溶性鸟苷酸环化酶的激活似乎是由过氧化氢酶的化合物I或该物种产生的介质引起的。
