Khalid Muhammad, Hassani Danial, Bilal Muhammad, Asad Fayaz, Huang Danfeng
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Bot Stud. 2017 Aug 16;58(1):35. doi: 10.1186/s40529-017-0189-3.
This study evaluates the influences of bio fertilizers containing mycorrhizal fungi (Glomus fasciculatum, Glomus mosseae) individually or in combination with N-fixer (Azotobacter chroococcum), K solubilizer (Bacillus mucilaginous) and P solubilizer (Bacillus megaterium) on soil fertility and phytochemical levels of spinach.
Root colonization by mycorrhizal fungi was increased in the presence of bacterial inoculation in comparison to individual inoculation treatments. Inoculation of bio fertilizer containing mycorrhizal fungi and bacterial species considerably augmented the concentration of total phenolic compounds, flavonoids and phenolic acid contents. The 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging capacity of spinach was found to be positively coincided with flavonoid contents, while partially correlated with total phenolic compounds and phenolic acids. Further, the HPLC analysis showed that significantly higher antioxidant activity of spinach was correlated with quercetin contents and chlorogenic acid. Chlorophyll contents were also increased following the bio fertilization treatments.
Results revealed that these microbes are useful tool for improving health promoting compounds in spinach.
本研究评估了单独含有菌根真菌（束状球囊霉、摩西球囊霉）或与固氮菌（褐球固氮菌）、解钾菌（胶质芽孢杆菌）和解磷菌（巨大芽孢杆菌）组合的生物肥料对菠菜土壤肥力和植物化学物质水平的影响。
与单独接种处理相比，在细菌接种的情况下，菌根真菌的根部定殖增加。接种含有菌根真菌和细菌种类的生物肥料显著提高了总酚类化合物、黄酮类化合物和酚酸含量的浓度。发现菠菜的1,1-二苯基-2-苦基肼（DPPH）清除能力与黄酮类化合物含量呈正相关，而与总酚类化合物和酚酸部分相关。此外，高效液相色谱分析表明，菠菜显著更高的抗氧化活性与槲皮素含量和绿原酸相关。生物施肥处理后叶绿素含量也增加。
结果表明，这些微生物是提高菠菜中健康促进化合物的有用工具。
