Lu Qi, Pan Zhuo-Hua
Department of Anatomy and Cell Biology, Wayne State University School of Medicine, 540 E. Canfield Avenue, Detroit, MI, 48201, USA.
Department of Ophthalmology, Kresge Eye Institute, Wayne State University School of Medicine, Detroit, MI, USA.
Methods Mol Biol. 2017;1642:181-194. doi: 10.1007/978-1-4939-7169-5_12.
The retina is a thin neural tissue sitting on the backside of the eye, composed of light-sensing cells, interneurons, and output ganglion neurons. The latter send electrical signals to higher visual centers in the brain. Transgenic mouse lines are becoming one of the most valuable mammalian animal models for the study of visual signal processing within the retina. Especially, the generation of Cre recombinase transgenic mouse lines provides a powerful tool for genetic manipulation. A key step for the utilization of transgenic lines is the characterization of their transgene expression patterns in the retina. Here we describe a standard protocol for characterizing the expression pattern of the Cre recombinase or fluorescent proteins in the retina with an immunohistochemical approach.
视网膜是位于眼球后部的一层薄神经组织，由光感细胞、中间神经元和输出神经节神经元组成。后者将电信号发送至大脑中的高级视觉中枢。转基因小鼠品系正成为研究视网膜内视觉信号处理的最有价值的哺乳动物动物模型之一。特别是，Cre重组酶转基因小鼠品系的产生为基因操作提供了一个强大的工具。利用转基因品系的关键步骤是表征其在视网膜中的转基因表达模式。在此，我们描述一种用免疫组织化学方法表征视网膜中Cre重组酶或荧光蛋白表达模式的标准方案。
