Aktas M, Ozubek S
Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey.
Med Vet Entomol. 2018 Mar;32(1):78-83. doi: 10.1111/mve.12261. Epub 2017 Aug 16.
This study investigated the transmission of Anaplasma platys by Rhipicephalus sanguineus sensu lato (Ixodida: Ixodidae). Engorged nymphs (n = 404) removed from A. platys-infected dogs were incubated at 28 °C until moulting. Unfed adults were obtained and divided into 48 pools. Unfed ticks collected from the grounds of the dog shelter, comprising 1800 larvae (n = 18 pools), 3100 nymphs (n = 62 pools) and 85 adults (n = 10 pools, including three male and seven female pools) were sorted into 90 pools. All pools were screened by polymerase chain reaction for the 16S rRNA gene of A. platys. Of 48 pools of unfed adults obtained from engorged nymphs, 12 were positive for A. platys; the infection rate maximum likelihood estimate (MLE) was 3.36 [95% confidence interval (CI) 1.84-5.68]. Anaplasma platys was detected in five of 23 male pools (MLE 2.82, 95% CI 1.06-6.20) and seven of 25 female pools (MLE 3.83, 95% CI 1.72-7.57). Of seven pools of unfed adult females collected from the shelter grounds, one was positive for A. platys (MLE 1.74, 95% CI 0.11-8.22). Among 62 unfed nymph pools, eight were infected with A. platys (MLE 0.27, 95% CI 0.13-0.52). No A. platys DNA was detected in the larva pools. The present results reveal molecular evidence for the trans-stadial transmission of A. platys by R. sanguineus s.l.
本研究调查了血红扇头蜱（狭义）（蜱螨目：硬蜱科）传播血小板无形体的情况。从感染血小板无形体的犬只身上采集饱血若蜱（n = 404），在28℃下孵育至蜕皮。获得未进食的成蜱并分成48组。从犬舍地面收集的未进食蜱，包括1800只幼虫（n = 18组）、3100只若蜱（n = 62组）和85只成蜱（n = 10组，包括3组雄性和7组雌性），被分成90组。所有组均通过聚合酶链反应筛选血小板无形体的16S rRNA基因。从饱血若蜱获得的48组未进食成蜱中，12组血小板无形体呈阳性；感染率最大似然估计值（MLE）为3.36 [95%置信区间（CI）1.84 - 5.68]。在23组成蜱雄性组中的5组检测到血小板无形体（MLE 2.82，95% CI 1.06 - 6.20），在25组成蜱雌性组中的7组检测到（MLE 3.83，95% CI 1.72 - 7.57）。从犬舍地面收集的7组未进食成年雌性蜱中，1组血小板无形体呈阳性（MLE 1.74，95% CI 0.11 - 8.22）。在62组未进食若蜱组中，8组感染了血小板无形体（MLE 0.27，95% CI 0.13 - 0.52）。在幼虫组中未检测到血小板无形体DNA。目前的结果揭示了血红扇头蜱狭义种传播血小板无形体经变态期传播的分子证据。
