Jiangsu Provincial Key Laboratory for Interventional Medical Devices, Huaiyin Institute of Technology , Huai'an 223003, China.
Stroke Biological Recovery Laboratory, Spaulding Rehabilitation Hospital, Harvard Medical School , Charlestown, Massachusetts 02129, United States.
ACS Appl Mater Interfaces. 2017 Sep 13;9(36):30373-30386. doi: 10.1021/acsami.7b08516. Epub 2017 Aug 28.
Surface biomimetic modification with extra-cellular matrix (ECM)-derived biomolecules is an emerging potential method of accelerating the healing of vascular stent lesions. However, insufficient capacity of the constructed biofunctional layer in maintaining its long-term efficiency and preventing thrombus and neointimal hyperplasia continue to be major limitations in clinical application. On the basis of the structure and function of ECM, in this study, we constructed a novel stromal cell-derived factor-1α (SDF-1α)/laminin-loaded nanocoating on the 316L stainless steel (SS) surface to provide improved function in modulation of vascular remodeling. The modified surface was found to control delivery of biomolecules and exhibit promising potential to provide stage-adjusted treatment after injury. An in vitro biocompatibility study suggested that the constructed layer may effectively prevent thrombosis formation by inhibiting platelet adhesion and activation, while accelerating endothelium regeneration by inducing endothelial cell (EC) migration and endothelial progenitor cell (EPC) aggregation. An in vivo animal test further demonstrated that the nanocoating may prevent thrombus and neointimal hyperplasia after implantation for 3 months. Therefore, the ECM-inspired nanocoating described in this study is a promising novel approach for vascular stent surface modification.
表面仿生修饰与细胞外基质（ECM）衍生的生物分子是加速血管支架损伤愈合的一种新兴的有潜力的方法。然而，构建的生物功能层在维持其长期效率和防止血栓和新生内膜增生方面的能力不足，仍然是临床应用的主要限制。基于 ECM 的结构和功能，本研究构建了一种新型基质细胞衍生因子-1α（SDF-1α）/层粘连蛋白负载的纳米涂层，以改善对血管重塑的调节功能。改性表面被发现可以控制生物分子的释放，并表现出在损伤后提供阶段性调整治疗的潜力。体外生物相容性研究表明，该构建层可以通过抑制血小板黏附和激活，有效防止血栓形成，同时通过诱导内皮细胞（EC）迁移和内皮祖细胞（EPC）聚集，促进内皮再生。体内动物实验进一步证明，纳米涂层可以在植入 3 个月后防止血栓和新生内膜增生。因此，本研究中描述的基于 ECM 的纳米涂层是一种有前途的血管支架表面修饰的新方法。
