Department of Psychiatry and Psychosomatics, Division of Psychiatry I, Medical University of Innsbruck, Innsbruck, Austria.
Department of Psychiatry and Psychotherapy A, General Hospital, Hall, Austria.
Int Psychogeriatr. 2018 Jan;30(1):147-152. doi: 10.1017/S1041610217001612. Epub 2017 Aug 17.
Alzheimer´s disease (AD) is a severe neurodegenerative brain disorder characterized by beta-amyloid plaques, Tau pathology, inflammation, neurodegeneration, and cerebrovascular dysfunction. Besides that, alterations in monocytes and platelets have been reported in the blood of Alzheimer patients. In the present study, we measured circulating levels of platelet-monocyte aggregates in EDTA blood of cognitively healthy participants and 40 AD patients, and examined their changes induced by stimulation with beta-amyloid peptides. We measured CD14, CD62P, and CD42a using fluorescence-activated cell scanning (FACS) analysis. Our data show that the levels of circulating monocyte-platelet aggregates were not different between healthy controls and AD patients. However, incubation with beta-amyloid-40, -42, and pyroglutamate-beta-amyloid increased the platelet-monocyte aggregation in healthy subjects, but not AD patients. Our data conclude that the interaction between monocytes and platelets is not altered in whole blood of AD patients, but their sensitivity toward beta-amyloid peptides is decreased. There might be a critical link between the interaction of platelets and monocytes in AD, which has to be explored in further studies.
阿尔茨海默病（AD）是一种严重的神经退行性脑疾病，其特征是β-淀粉样斑块、Tau 病理学、炎症、神经退行性变和脑血管功能障碍。此外，阿尔茨海默病患者血液中的单核细胞和血小板发生改变已经有报道。在本研究中，我们测量了 EDTA 血液中认知健康参与者和 40 名 AD 患者的血小板-单核细胞聚集物的循环水平，并检查了它们在β-淀粉样肽刺激下的变化。我们使用荧光激活细胞扫描（FACS）分析测量 CD14、CD62P 和 CD42a。我们的数据表明，循环单核细胞-血小板聚集物的水平在健康对照组和 AD 患者之间没有差异。然而，β-淀粉样肽 40、42 和焦谷氨酸-β-淀粉样肽孵育增加了健康受试者的血小板-单核细胞聚集，但 AD 患者没有。我们的数据得出结论，AD 患者全血中单核细胞和血小板之间的相互作用没有改变，但它们对β-淀粉样肽的敏感性降低。血小板和单核细胞在 AD 中的相互作用之间可能存在关键联系，这需要在进一步的研究中进行探索。
