a Laboratory of Molecular Genetics of Microorganisms, Institute of Molecular Genetics , Russian Academy of Sciences , Moscow , Russia.
b Molecular Biology Department, Biological Faculty , Moscow State University , Moscow , Russia.
RNA Biol. 2017 Dec 2;14(12):1678-1682. doi: 10.1080/15476286.2017.1356568. Epub 2017 Sep 13.
The σ subunit of bacterial RNA polymerase is required for promoter recognition during transcription initiation but may also regulate transcription elongation. The principal σ subunit of Escherichia coli was shown to travel with RNA polymerase and induce transcriptional pausing at promoter-like motifs, with potential regulatory output. We recently demonstrated that an alternative σ subunit can also induce RNA polymerase pausing. Here, we outline proposed regulatory roles of σ-dependent pausing in bacteria and discuss possible interplay between alternative σ variants and regulatory factors during transcription elongation.
细菌 RNA 聚合酶的 σ 亚基对于转录起始过程中的启动子识别是必需的，但它也可能调节转录延伸。已经证明大肠杆菌的主要 σ 亚基与 RNA 聚合酶一起移动，并在启动子样基序处诱导转录暂停，从而产生潜在的调节输出。我们最近证明，另一种 σ 亚基也可以诱导 RNA 聚合酶暂停。在这里，我们概述了 σ 依赖性暂停在细菌中的调节作用，并讨论了在转录延伸过程中替代 σ 变体和调节因子之间可能的相互作用。
