Koller Ulrich, Waldstein Wenzel, Krenn Veit, Windhager Reinhard, Boettner Friedrich
Department of Orthopaedics, Vienna General Hospital, Medical University of Vienna, Vienna, Austria.
Medizinisches Versorgungszentrum für Histologie, Zytologie und Molekulare Diagnostik Trier, Trier, Germany.
J Orthop Res. 2018 Mar;36(3):841-846. doi: 10.1002/jor.23708. Epub 2017 Aug 29.
The study analyzed the influence of synovitis on the histological and biomechanical properties of lateral-compartment cartilage. In a prospective cohort study, 84 patients (100 knees) with varus deformity of the knee were included. Osteochondral samples from the distal lateral femur underwent biomechanical and histologic analysis. Synovial tissue was sampled for histological (chronic synovitis score) and immunohistochemical evaluation of the degree of synovitis. CD15 (neutrophils), Ki-67 (dividing cells), and CD68 (macrophages) were tested in all synovial samples. While the histological synovitis score did not correlate with the degree of cartilage degeneration (histological OARSI grades), both CD15 (r = 0.297, p = 0.006) and Ki-67 (r = 0.249, p = 0.023) correlated with histological OARSI grades. There was a weak negative correlation of CD15 with biomechanical properties of cartilage of the distal lateral femur (aggregate modulus (Ha): r = -0.125; p = 0.257; dynamic modulus (DM): r = -0.216; p = 0.048). No correlations were observed for Ki-67 and CD68. In addition, biomechanical properties were inferior in knees with a CD15 of >8/high power field compared to knees with a CD15 of ≤8/high power field (Ha: p = 0.031, d = 0.46; DM: p = 0.005, d = 0.68). The study demonstrates an association of increased inflammatory activity with advanced cartilage degeneration. Lateral-compartment cartilage in knees with elevated synovial CD15 counts has a reduced ability to withstand compressive loads. CD15 might serve as an indicator for inferior biomechanical cartilage properties. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:841-846, 2018.
该研究分析了滑膜炎对外侧间室软骨组织学和生物力学特性的影响。在一项前瞻性队列研究中，纳入了84例膝内翻畸形患者（100个膝关节）。取自股骨外侧远端的骨软骨样本进行了生物力学和组织学分析。采集滑膜组织进行组织学（慢性滑膜炎评分）及滑膜炎程度的免疫组化评估。对所有滑膜样本检测CD15（中性粒细胞）、Ki-67（增殖细胞）和CD68（巨噬细胞）。虽然组织学滑膜炎评分与软骨退变程度（组织学OARSI分级）无关，但CD15（r = 0.297，p = 0.006）和Ki-67（r = 0.249，p = 0.023）均与组织学OARSI分级相关。CD15与股骨外侧远端软骨的生物力学特性呈弱负相关（聚集模量（Ha）：r = -0.125；p = 0.257；动态模量（DM）：r = -0.216；p = 0.048）。未观察到Ki-67和CD68有相关性。此外，与CD15≤8/高倍视野的膝关节相比，CD15>8/高倍视野的膝关节生物力学特性较差（Ha：p = 0.031，d = 0.46；DM：p = 0.005，d = 0.68）。该研究表明炎症活动增加与软骨退变加重有关。滑膜CD15计数升高的膝关节外侧间室软骨承受压缩负荷的能力降低。CD15可能是软骨生物力学特性较差的一个指标。©2017骨科研究学会。由威利期刊公司出版。《矫形外科学研究》2018年第36卷：841 - 846页
