用于评估金属硫蛋白的汞-203法：铜、汞、氧、银和硒的干扰

The mercury-203 method for evaluating metallothioneins: interference by copper, mercury, oxygen, silver and selenium.

作者信息

Lobel P B, Payne J F

出版信息

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1987;86(1):37-9. doi: 10.1016/0742-8413(87)90140-x.

Abstract

Metallothioneins are low molecular weight proteins rich in sulfhydryl groups (cysteinyl) which readily bind various heavy metal cations, e.g. cadmium, copper, gold, mercury, silver and zinc. Mercury has a particular affinity for sulfhydryl groups and mercury-203 has been used as the basis of a rapid, sensitive, radiometric assay for metallothionein. The potential of 16 metals and oxygen for interfering with this test was examined. The mercury-203 test appears to be sensitive to the presence of copper, mercury, oxygen, selenium and silver.

摘要

金属硫蛋白是一类富含巯基（半胱氨酰）的低分子量蛋白质，它能轻易结合各种重金属阳离子，如镉、铜、金、汞、银和锌。汞对巯基具有特殊的亲和力，汞-203已被用作一种快速、灵敏的金属硫蛋白放射性测定法的基础。研究了16种金属和氧干扰该测试的可能性。汞-203测试似乎对铜、汞、氧、硒和银的存在敏感。