Jiang Li-Peng, Zhu Zhi-Tu, Zhang Yue, He Chun-Yan
Department of Radiation Oncology, Jinzhou, China.
Department of Oncology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.
Cell Physiol Biochem. 2017;42(6):2220-2229. doi: 10.1159/000479996. Epub 2017 Aug 16.
The present study sought to explore the role of microRNA-330 (miR-330) in predicting the radiation response and prognosis of patients with brain metastasis (BM) from lung cancer (LC).
Patients with BM from LC were identified and classified into radiation-sensitive and radiation-resistant groups according to the overall survival rate, local and distant recurrence rate after conventional whole-brain radiation therapy. Quantitative realtime polymerase chain reaction (qRT-PCR) was used to detect miR-330 expression in serum. Receiver operating characteristic (ROC) curves were used to evaluate the prognostic value of miR-330 for the radiation sensitivity of brain metastasis from LC. Related clinical factors for radiation sensitivity were assessed by logistic regression analysis, and a survival analysis was conducted using COX regression and the Kaplan-Meier method.
MiR-330 exhibited lower expression in the radiation-sensitive group than in the radiation-resistant group. The area under the ROC curve of miR-330 for predicting radiation sensitivity was 0.898 (optimal cut-off value, 0.815), with a sensitivity of 71.7% and a specificity of 90.1%. After radiation therapy, patients with low miR-330 expression, compared to patients with high miR-330 expression, displayed a lower survival rate and a median survival time. MiR-330 expression was correlated with extracranial metastasis, maximum BM diameter, tumor-node-metastasis (TNM) stage and node (N) stage. Logistic regression and COX regression analyses revealed that extracranial metastasis, TNM stage, N stage and miR-330 expression were factors that influenced both radiation sensitivity and individual prognostic factors in patients with BM from LC.
These findings indicate that the downregulation of miR-330 correlates with radiation sensitivity and poor prognosis in patients with BM from LC.
本研究旨在探讨微小RNA-330（miR-330）在预测肺癌脑转移（BM）患者的放射反应和预后中的作用。
识别出肺癌脑转移患者，并根据常规全脑放射治疗后的总生存率、局部和远处复发率将其分为放射敏感组和放射抵抗组。采用定量实时聚合酶链反应（qRT-PCR）检测血清中miR-330的表达。采用受试者工作特征（ROC）曲线评估miR-330对肺癌脑转移放射敏感性的预后价值。通过逻辑回归分析评估放射敏感性的相关临床因素，并使用COX回归和Kaplan-Meier方法进行生存分析。
miR-330在放射敏感组中的表达低于放射抵抗组。miR-330预测放射敏感性的ROC曲线下面积为0.898（最佳截断值为0.815），敏感性为71.7%，特异性为90.1%。放射治疗后，与miR-330高表达患者相比，miR-330低表达患者的生存率和中位生存时间较低。miR-330表达与颅外转移、最大脑转移瘤直径、肿瘤-淋巴结-转移（TNM）分期和淋巴结（N）分期相关。逻辑回归和COX回归分析显示，颅外转移、TNM分期、N分期和miR-330表达是影响肺癌脑转移患者放射敏感性和个体预后因素的因素。
这些发现表明，miR-330的下调与肺癌脑转移患者的放射敏感性和不良预后相关。
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