基质细胞衍生因子-1a（SDF-1a）在白消安损伤大鼠生精中的作用。
Role of stromal derived factor-1a (SDF-1a) for spermatogenesis of busulfan-injured rats.
机构信息
Department of Anatomical Sciences, Tehran University of Medical Sciences, Tehran, Iran.
Department of Anatomy, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
出版信息
Reprod Toxicol. 2017 Oct;73:142-148. doi: 10.1016/j.reprotox.2017.08.006. Epub 2017 Aug 14.
SDF-1a is a member of CXC chemokine family that plays a crucial role in stem cell migration, cell apoptosis and development. The role of intra-scrotal administration of SDF-1a in spermatogenesis of busulfan-treated rats was investigated in this study. Two injections of busulfan (15mg/kg) with a 14days interval between were given intraperitoneally to male Wistar rats. Rats were then treated for seven days with 500ng/mL SDF-1a. Real-time PCR and immunohistochemistry were performed for evaluation of various cell markers for proliferation and spermatogenesis, and sperm parameters were assessed. In the SDF-1a group, there was a significant increase in testis weight, sperm count and viability. DAZL, DDX4, and TP2 showed increased expression levels in the SDF-1a group. PCNA and BrdU revealed highest expression rates in the SDF-1a group (p≤0.0001). These findings showed the protective role of SDF-1a in busulfan-induced testis injury most likely through stimulation of SSCs proliferation.
SDF-1a 是 CXC 趋化因子家族的一员，在干细胞迁移、细胞凋亡和发育中起着关键作用。本研究探讨了 SDF-1a 经睾丸内给药在白消安处理大鼠生精中的作用。雄性 Wistar 大鼠腹腔内给予两次白消安（15mg/kg），间隔 14 天。然后，大鼠用 500ng/ml SDF-1a 治疗 7 天。通过实时 PCR 和免疫组织化学评估各种增殖和生精细胞标志物，并评估精子参数。在 SDF-1a 组中，睾丸重量、精子计数和活力均显著增加。DAZL、DDX4 和 TP2 在 SDF-1a 组中的表达水平升高。PCNA 和 BrdU 在 SDF-1a 组中显示出最高的表达率（p≤0.0001）。这些发现表明 SDF-1a 通过刺激 SSCs 增殖在白消安诱导的睾丸损伤中发挥保护作用。
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