Department of Plant Sciences, School of Life Sciences, Manipal University, Manipal, Karnataka, India.
Department of Biophysics, School of Life Sciences, Manipal University, Manipal, Karnataka, India.
J Photochem Photobiol B. 2017 Sep;174:333-341. doi: 10.1016/j.jphotobiol.2017.08.017. Epub 2017 Aug 10.
The present investigation was designed to analyze the influence of Helium-Neon (He-Ne 632.8nm) laser irradiation on defense enzymes, proline content and in vitro responses of callus induction, shoot initiation and on plantlet regeneration potential of brinjal. The seeds of Mattu Gulla (Solanum melongena L.) were irradiated with 20, 25 and 30J/cm of He-Ne laser followed by surface sterilization and sprouted on Murashige and Skoog medium without plant growth regulators. The activity of defense enzymes, proline content and the organogenetic potential of hypocotyl, leaf and shoot tip explants were determined from thirty day old seedlings. During seed germination, most of the seedlings showed normal two cotyledons whereas small number of seedlings showed tricotyledonous at 20J/cm treatment and no other morphological abnormalities were observed during further growth and development. There was no substantial variation was noted in both β-1,3-glucanase and chitinase activity as well as proline content which proves the He-Ne laser irradiation does not causes any stresses for the plant. The in vitro culture of hypocotyl, leaf and shoot tip explants from laser irradiated seedlings showed differential responses as compared to un-irradiated control. The laser induced enhancement of callus induction, growth rate of callus tissues and shoot tip, percentage of responses of shoot and root initiation, days to shoot and root initiation, shoots formed per callus, number of roots per shoots, length of roots and nuclear DNA content of in vitro raised plants were evaluated. Among the tested laser doses (20, 25 and 30J/cm), 25J/cm showed significant biostimulatory effect over un-irradiated control seedlings. The present observations reveal and endorsed our earlier reports with substantial enhancement of in vitro and ex vitro by He-Ne laser irradiation.
本研究旨在分析氦氖（He-Ne 632.8nm）激光照射对防御酶、脯氨酸含量以及茄子愈伤组织诱导、芽起始和植株再生潜力的体外反应的影响。用 20、25 和 30J/cm 的 He-Ne 激光照射 Mattu Gulla（茄子 Solanum melongena L.）种子，然后进行表面消毒，并在没有植物生长调节剂的 Murashige 和 Skoog 培养基上发芽。从 30 天大的幼苗中测定防御酶的活性、脯氨酸含量和下胚轴、叶片和茎尖外植体的器官发生潜力。在种子发芽期间，大多数幼苗表现出正常的两片子叶，而在 20J/cm 处理下，少数幼苗表现出三叶性，在进一步的生长和发育过程中没有观察到其他形态异常。β-1,3-葡聚糖酶和几丁质酶活性以及脯氨酸含量均无明显变化，这证明 He-Ne 激光照射不会对植物造成任何压力。与未照射对照相比，激光照射幼苗的下胚轴、叶片和茎尖外植体的体外培养表现出不同的反应。激光诱导增强了愈伤组织诱导、愈伤组织组织的生长速度和茎尖、芽和根起始的百分比、芽和根起始的天数、每个愈伤组织形成的芽数、每个芽的根数、根的长度和体外培养植物的核 DNA 含量。在测试的激光剂量（20、25 和 30J/cm）中，25J/cm 对未照射对照幼苗表现出显著的生物刺激作用。目前的观察结果证实并支持了我们之前的报告，即 He-Ne 激光照射可显著增强体外和外体的生长。
