Briz Victor, Restivo Leonardo, Pasciuto Emanuela, Juczewski Konrad, Mercaldo Valentina, Lo Adrian C, Baatsen Pieter, Gounko Natalia V, Borreca Antonella, Girardi Tiziana, Luca Rossella, Nys Julie, Poorthuis Rogier B, Mansvelder Huibert D, Fisone Gilberto, Ammassari-Teule Martine, Arckens Lutgarde, Krieger Patrik, Meredith Rhiannon, Bagni Claudia
KU Leuven, Department of Neurosciences, Leuven Research Institute for Neuroscience and Disease (LIND), KU Leuven, Leuven, 3000, Belgium.
VIB Center for Brain & Disease Research, Department of Neurosciences KU Leuven, Leuven, 3000, Belgium.
Nat Commun. 2017 Aug 17;8(1):293. doi: 10.1038/s41467-017-00311-2.
The brain cytoplasmic (BC1) RNA is a non-coding RNA (ncRNA) involved in neuronal translational control. Absence of BC1 is associated with altered glutamatergic transmission and maladaptive behavior. Here, we show that pyramidal neurons in the barrel cortex of BC1 knock out (KO) mice display larger excitatory postsynaptic currents and increased spontaneous activity in vivo. Furthermore, BC1 KO mice have enlarged spine heads and postsynaptic densities and increased synaptic levels of glutamate receptors and PSD-95. Of note, BC1 KO mice show aberrant structural plasticity in response to whisker deprivation, impaired texture novel object recognition and altered social behavior. Thus, our study highlights a role for BC1 RNA in experience-dependent plasticity and learning in the mammalian adult neocortex, and provides insight into the function of brain ncRNAs regulating synaptic transmission, plasticity and behavior, with potential relevance in the context of intellectual disabilities and psychiatric disorders.Brain cytoplasmic (BC1) RNA is a non-coding RNA that has been implicated in translational regulation, seizure, and anxiety. Here, the authors show that in the cortex, BC1 RNA is required for sensory deprivation-induced structural plasticity of dendritic spines, as well as for correct sensory learning and social behaviors.
脑细胞质（BC1）RNA是一种参与神经元翻译控制的非编码RNA（ncRNA）。BC1的缺失与谷氨酸能传递改变和适应不良行为有关。在此，我们表明，BC1基因敲除（KO）小鼠桶状皮质中的锥体神经元在体内表现出更大的兴奋性突触后电流和自发活动增加。此外，BC1 KO小鼠的棘突头部和突触后致密物增大，谷氨酸受体和PSD-95的突触水平增加。值得注意的是，BC1 KO小鼠在对触须剥夺的反应中表现出异常的结构可塑性，纹理新物体识别受损和社交行为改变。因此，我们的研究突出了BC1 RNA在哺乳动物成年新皮质中依赖经验的可塑性和学习中的作用，并为调节突触传递、可塑性和行为的脑ncRNAs的功能提供了见解，这在智力残疾和精神疾病的背景下可能具有相关性。脑细胞质（BC1）RNA是一种与翻译调节、癫痫发作和焦虑有关的非编码RNA。在此，作者表明，在皮质中，BC1 RNA是感觉剥夺诱导的树突棘结构可塑性以及正确的感觉学习和社交行为所必需的。
