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破坏植物特异性 CFS1 基因会损害自噬体的周转，并触发依赖于 EDS1 的细胞死亡。

Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death.

机构信息

Botanik III, Biocenter, Universtiy of Cologne, Zülpicher Str. 47B, 50674, Cologne, Germany.

Department of Biomedical Science, The University of Sheffield, Western Bank Sheffield, S10 2TN, United Kingdom.

出版信息

Sci Rep. 2017 Aug 17;7(1):8677. doi: 10.1038/s41598-017-08577-8.

DOI:10.1038/s41598-017-08577-8
PMID:28819237
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5561093/
Abstract

Cell death, autophagy and endosomal sorting contribute to many physiological, developmental and immunological processes in plants. They are mechanistically interconnected and interdependent, but the molecular basis of their mutual regulation has only begun to emerge in plants. Here, we describe the identification and molecular characterization of CELL DEATH RELATED ENDOSOMAL FYVE/SYLF PROTEIN 1 (CFS1). The CFS1 protein interacts with the ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT I (ESCRT-I) component ELCH (ELC) and is localized at ESCRT-I-positive late endosomes likely through its PI3P and actin binding SH3YL1 Ysc84/Lsb4p Lsb3p plant FYVE (SYLF) domain. Mutant alleles of cfs1 exhibit auto-immune phenotypes including spontaneous lesions that show characteristics of hypersensitive response (HR). Autoimmunity in cfs1 is dependent on ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)-mediated effector-triggered immunity (ETI) but independent from salicylic acid. Additionally, cfs1 mutants accumulate the autophagy markers ATG8 and NBR1 independently from EDS1. We hypothesize that CFS1 acts at the intersection of autophagosomes and endosomes and contributes to cellular homeostasis by mediating autophagosome turnover.

摘要

细胞死亡、自噬和内体分选在植物的许多生理、发育和免疫过程中发挥作用。它们在机制上相互关联和相互依存，但它们相互调节的分子基础在植物中才刚刚开始出现。在这里，我们描述了 CELL DEATH RELATED ENDOSOMAL FYVE/SYLF PROTEIN 1 (CFS1) 的鉴定和分子特征。CFS1 蛋白与内体分选复合物必需的运输 I (ESCRT-I) 成分 ELCH (ELC) 相互作用，并定位于 ESCRT-I 阳性晚期内体，可能通过其 PI3P 和肌动蛋白结合 SH3YL1 Ysc84/Lsb4p Lsb3p 植物 FYVE (SYLF) 结构域。cfs1 的突变等位基因表现出自免疫表型，包括自发病变，表现出过敏反应 (HR) 的特征。cfs1 的自身免疫依赖于增强疾病敏感性 1 (EDS1) 介导的效应物触发的免疫 (ETI)，但与水杨酸无关。此外，cfs1 突变体独立于 EDS1 积累自噬标记物 ATG8 和 NBR1。我们假设 CFS1 作用于自噬体和内体的交点，并通过介导自噬体周转来促进细胞内稳态。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8a4/5561093/9f4dc5ab62dc/41598_2017_8577_Fig7_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8a4/5561093/8cbb90345ee4/41598_2017_8577_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8a4/5561093/9f4dc5ab62dc/41598_2017_8577_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8a4/5561093/19fa23ffd4be/41598_2017_8577_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8a4/5561093/743531de0f90/41598_2017_8577_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8a4/5561093/a7ddced049dc/41598_2017_8577_Fig3_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8a4/5561093/2e6f2c677abf/41598_2017_8577_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8a4/5561093/8cbb90345ee4/41598_2017_8577_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8a4/5561093/9f4dc5ab62dc/41598_2017_8577_Fig7_HTML.jpg

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