Alton K B, Petruzzi R F, Patrick J E
J Chromatogr. 1987 Jan 9;385:249-59. doi: 10.1016/s0021-9673(01)94637-0.
A high-performance liquid chromatographic assay for the quantitative determination of azatadine and a base (1 M sodium hydroxide) hydrolyzable conjugate of azatadine in human urine has been developed. Reversed-phase separation of azatadine and the internal standard, 8-chloroazatadine, was accomplished on a 300 X 3.9 mm I.D. mu Bondapak CN column. Following liquid-liquid extraction from urine, azatadine was quantitatively determined by UV detection at 214 nm. No interferences were observed in the extracts obtained from drug-free urine. Detector response (peak area ratio) was linear from 10 to 2500 ng/ml. This method has been shown to provide accurate and precise determinations of the unchanged and hydrolyzed drug in human urine, following the twice daily oral administration (1-2 mg) of azatadine maleate.
已开发出一种高效液相色谱分析法，用于定量测定人尿中阿扎他定及其可被碱（1 M氢氧化钠）水解的阿扎他定共轭物。阿扎他定和内标物8-氯阿扎他定在300×3.9 mm内径的μ Bondapak CN柱上进行反相分离。从尿液中进行液-液萃取后，通过在214 nm处的紫外检测对阿扎他定进行定量测定。从无药尿液中获得的提取物未观察到干扰。检测器响应（峰面积比）在10至2500 ng/ml范围内呈线性。该方法已被证明在每日两次口服（1 - 2 mg）马来酸阿扎他定后，能够准确、精确地测定人尿中未变化和水解的药物。
