使用候选干细胞标志物p75神经营养因子受体（p75NTR）进行循环肿瘤细胞的流式细胞术检测。

Flow Cytometric Detection of Circulating Tumor Cells Using a Candidate Stem Cell Marker, p75 Neurotrophin Receptor (p75NTR).

作者信息

Okumura Tomoyuki, Yamaguchi Tetsuji, Watanabe Toru, Nagata Takuya, Shimada Yutaka

机构信息

Department of Surgery and Science, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, 2630 Sugitani, Toyama City, Toyama, 930-0194, Japan.

Department of Nanobio Drug Discovery, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-Shimo-Adachi-cho, Sakyo-ku, Kyoto, 606-8501, Japan.

出版信息

Methods Mol Biol. 2017;1634:211-217. doi: 10.1007/978-1-4939-7144-2_18.

DOI:10.1007/978-1-4939-7144-2_18

PMID:28819854

Abstract

The most widely studied detection for circulating tumor cells (CTCs) in peripheral blood of cancer patients has been based on immunomagnetic enrichment using antibodies against epithelial cell adhesion molecule (EpCAM), which is overexpressed in epithelial cells. A neurotrophin receptor p75 (p75NTR) is expressed in a candidate stem cell fraction in esophageal squamous cell carcinoma (ESCC), which shows significantly higher colony formation, enhanced tumor formation in mice, along with strong expression of epithelial mesenchymal transition-related genes. Here, we describe a method to detect CTCs in ESCC based on the combined expression of EpCAM and p75NTR using flow cytometry, demonstrating the feasibility of expression analysis of multiple cell surface markers in viable cells.

摘要

针对癌症患者外周血中循环肿瘤细胞（CTC）的检测，研究最为广泛的方法是基于使用抗上皮细胞粘附分子（EpCAM）抗体的免疫磁珠富集技术，EpCAM在上皮细胞中过度表达。神经营养因子受体p75（p75NTR）在食管鳞状细胞癌（ESCC）的候选干细胞组分中表达，该组分显示出明显更高的集落形成能力、在小鼠体内更强的肿瘤形成能力，以及上皮-间质转化相关基因的高表达。在此，我们描述了一种基于EpCAM和p75NTR联合表达，利用流式细胞术检测ESCC中CTC的方法，证明了对活细胞中多种细胞表面标志物进行表达分析的可行性。