Faculty of Chemical & Process Engineering, Warsaw University of Technology, Warynskiego 1, 00-645 Warsaw, Poland.
Institute of Biochemistry & Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106 Warsaw, Poland.
Nanomedicine (Lond). 2017 Sep;12(18):2183-2197. doi: 10.2217/nnm-2017-0112. Epub 2017 Aug 18.
Developing pH-responsive multiple emulsion platforms for effective glioblastoma multiforme therapy with reduced toxicity, a drug release study and modeling.
MATERIALS & METHODS: Cancer cell line: U87 MG, multiple emulsions with pH-responsive biopolymer and encapsulated doxorubicin (DOX); preparation of multiple emulsions in a Couette-Taylor flow biocontactor, in vitro release study of DOX (fluorescence intensity analysis), in vitro cytotoxicity study (alamarBlue cell viability assay) and numerical simulation of DOX release rates.
The multiple emulsions offered a high DOX encapsulation efficiency (97.4 ± 1%) and pH modulated release rates of a drug. Multiple emulsions with a low concentration of DOX (0.02 μM) exhibited broadly advanced cell (U87 MG) cytotoxicity than free DOX solution used at the same concentration.
Emulsion platforms could be explored for potential delivery of chemotherapeutics in glioblastoma multiforme therapy.
开发 pH 响应型多重乳液平台，以降低毒性的方式有效治疗多形性胶质母细胞瘤，进行药物释放研究和建模。
癌细胞系：U87 MG，具有 pH 响应性生物聚合物和包封的阿霉素（DOX）的多重乳液；在 Couette-Taylor 流生物接触器中制备多重乳液，DOX 的体外释放研究（荧光强度分析），体外细胞毒性研究（alamarBlue 细胞活力测定）和 DOX 释放率的数值模拟。
多重乳液提供了高 DOX 包封效率（97.4±1%）和 pH 调节的药物释放速率。与相同浓度下使用的游离 DOX 溶液相比，低 DOX 浓度（0.02 μM）的多重乳液对细胞（U87 MG）表现出广泛的增效细胞毒性。
乳液平台可用于探索在多形性胶质母细胞瘤治疗中递送化疗药物的潜力。
