León María José, Aldeguer-Riquelme Borja, Antón Josefa, Sánchez-Porro Cristina, Ventosa Antonio
Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Department of Physiology, Genetics and Microbiology, University of Alicante, Alicante, Spain.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2947-2952. doi: 10.1099/ijsem.0.002053. Epub 2017 Aug 18.
A moderately halophilic bacterium, designated strain SP30T, was isolated from a solar saltern located in Santa Pola, Alicante, on the East coast of Spain. It was a Gram-stain-negative, strictly aerobic bacterium, able to grow in 7.5-25 % (w/v) NaCl and optimally in 12.5 % (w/v) NaCl. Phylogenetic analyses, based on 16S rRNA gene sequences, showed that the novel isolate is a member of the genus Spiribacter, with the most closely related species being Spiribacter roseus SSL50T (99.9 % sequence similarity) and Spiribacter curvatus UAH-SP71T (99.4 % sequence similarity). The 16S rRNA gene sequence similarity with the type species Spiribacter salinus M19-40T was 96.6 %. The DNA-DNA relatedness value between strain SP30T and S. roseus SSL50T and S. curvatus UAH-SP71T was 40 and 55 %, respectively; these values are lower than the 70 % threshold accepted for species delineation. The major fatty acids were C16:0, C18 : 1ω7c, C19 : 0 cyclo ω8c and C12 : 0. Similarly to other species of the genus Spiribacter, strain SP30Twas observed as curved rods and spiral cells. Metabolic versatility was reduced to the utilization of a few organic compounds as the sole carbon and energy sources, as with other members of Spiribacter. However, it differed in terms of colony pigmentation (brownish-yellow instead of pink) and in having a higher growth rate. Based on these data and on the phenotypic, genotypic and chemotaxonomic characterization, we propose the classification of strain SP30T as a novel species within the genus Spiribacter, with the name Spiribacter aquaticus sp. nov. The type strain is SP30T (=CECT 9238T=LMG 30005T).
从西班牙东海岸阿利坎特省圣波拉的一个太阳能盐场分离出一株中度嗜盐细菌，命名为SP30T菌株。它是革兰氏染色阴性、严格需氧的细菌，能在7.5 - 25%（w/v）NaCl中生长，最适生长NaCl浓度为12.5%（w/v）。基于16S rRNA基因序列的系统发育分析表明，该新分离菌株是螺旋杆菌属的成员，与玫瑰色螺旋杆菌SSL50T（序列相似性为99.9%）和弯曲螺旋杆菌UAH - SP71T（序列相似性为99.4%）亲缘关系最近。与模式种盐生螺旋杆菌M19 - 40T的16S rRNA基因序列相似性为96.6%。SP30T菌株与玫瑰色螺旋杆菌SSL50T和弯曲螺旋杆菌UAH - SP71T的DNA - DNA相关性值分别为40%和55%；这些值低于用于界定物种的70%阈值。主要脂肪酸为C16:0、C18 : 1ω7c、C19 : 0环ω8c和C12 : 0。与螺旋杆菌属的其他物种相似，SP30T菌株观察到为弯曲杆菌和螺旋状细胞。其代谢多样性降低，只能利用少数有机化合物作为唯一碳源和能源，与螺旋杆菌属的其他成员相同。然而，它在菌落色素沉着方面有所不同（呈棕黄色而非粉红色），且生长速率更高。基于这些数据以及表型、基因型和化学分类特征，我们提议将SP30T菌株分类为螺旋杆菌属内的一个新物种，命名为水生螺旋杆菌（Spiribacter aquaticus）。模式菌株为SP30T（=CECT 9238T = LMG 30005T）。
