College of Public Health, University of South China, Hengyang 421001, PR China.
College of Public Health, University of South China, Hengyang 421001, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jan 15;189:190-194. doi: 10.1016/j.saa.2017.08.022. Epub 2017 Aug 10.
We for the first time confirmed that the low concentrations of Ag(I) could induce a silver specific aptamer probe (SAP) from a random coil sequence form to G-quadruplex structure. Thereby, a novel highly sensitive fluorescence strategy for silver(I) assay was established. The designed multifunctional SAP could act as a recognition element for Ag(I) and a signal reporter. The use of such a SAP can ultrasensitively and selectively detect Ag(I), giving a detection limit down to 0.64nM. This is much lower than those reported by related literatures. This strategy has been applied successfully for the detection of Ag(I) in real samples, further proving its reliability. Taken together, the designed SAP is not only a useful recognition and signal probe for silver, but also gives a platform to study the interaction of monovalent cations with DNA.
我们首次证实，低浓度的 Ag(I) 可诱导随机卷曲序列形成银特异性适体探针 (SAP) 形成 G-四链体结构。由此，建立了一种用于银 (I) 分析的新型高灵敏度荧光策略。设计的多功能 SAP 可以作为 Ag(I)的识别元件和信号报告器。使用这种 SAP 可以超灵敏和选择性地检测 Ag(I)，检测限低至 0.64nM。这比相关文献报道的要低得多。该策略已成功应用于实际样品中 Ag(I)的检测，进一步证明了其可靠性。综上所述，设计的 SAP 不仅是一种有用的银识别和信号探针，还为研究单价阳离子与 DNA 的相互作用提供了一个平台。
