State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, China.
Soil and Water Science Department, University of Florida, Gainesville, FL 32611, USA.
Chemosphere. 2017 Nov;186:709-715. doi: 10.1016/j.chemosphere.2017.08.028. Epub 2017 Aug 8.
In this study, bioavailability of polychlorinated biphenyls (PCBs) in soil samples aged for various time intervals (7 days, 1 and 5 months) was assessed by in vivo tests using mice. The in vivo bioavailability of PCBs in soil ranged from 45% (PCB180 in soil aging for 5 month) to 119% (PCB52 in soil aging for 1 month), indicating that not all PCBs was available for absorption after ingestion of soil samples. The bioaccessibility was assessed using both physiologically-based extraction test (PBET) and Tenax improved PBET (TI-PBET). Acceptable in vivo-in vitro correlation (r = 0.70 and slope = 1.30 ± 0.20) was observed for TI-PBET, not for PBET. Due to dominant role played by Tenax and bile, the TI-PBET was further simplified to Tenax and Tenax-bile extraction methods. However, poor in vivo-in vitro correlation (r = 0.14 and 0.05) was observed for the two simplified methods, which may be attributed to the combined effect between sorption sink and components in PBET. Therefore, in order to simply TI-PBET or standardize in vitro methods, it is highly necessary to explore the mechanism about the interaction between in vitro method components and sorption sink, or to screen key factors for bioaccessibility results in the future studies.
本研究采用体内试验方法，以不同老化时间（7 天、1 个月和 5 个月）的土壤样品为研究对象，评估多氯联苯（PCBs）的生物可给性。结果表明，土壤中 PCB 的体内生物可给性范围为 45%（老化 5 个月的 PCB180）至 119%（老化 1 个月的 PCB52），这表明并非所有 PCB 在摄入土壤样品后都可被吸收。本研究还采用了生理相关提取试验（PBET）和 Tenax 改进 PBET（TI-PBET）评估了生物有效性。结果发现，TI-PBET 与体内试验具有较好的相关性（r=0.70，斜率=1.30±0.20），而 PBET 则没有。由于 Tenax 和胆汁在 TI-PBET 中起主导作用，因此进一步简化了 TI-PBET 为 Tenax 和 Tenax-胆汁提取方法。然而，两种简化方法与体内试验的相关性均较差（r=0.14 和 0.05），这可能是由于 PBET 中吸附阱和成分的共同作用所致。因此，为了简化 TI-PBET 或使体外方法标准化，非常有必要探索体外方法成分与吸附阱之间相互作用的机制，或在未来的研究中筛选生物有效性结果的关键因素。
