Zhao Bin, Zhao Zhihu, Sun Xiaolei, Zhang Yang, Guo Yue, Tian Peng, Ma Jianxiong, Ma Xinlong
Department of Orthopedics, Tianjin Medical University General Hospital, 154, Anshan Street, Heping District, Tianjin 300052, China; Institute of Orthopedics, Tianjin Hospital, Tianjin, China.
Institute of Orthopedics, Tianjin Hospital, Tianjin, China.
Biochem Biophys Res Commun. 2017 Oct 14;492(2):206-211. doi: 10.1016/j.bbrc.2017.08.050. Epub 2017 Aug 15.
The aim of this study was to analyze the effect of micro strain stress on the proliferation of endothelial progenitor cells in vitro by the MAPK-ERK1/2 signaling pathway.
In this study, we report the effect of micro strain stress on proliferation of endothelial progenitor cells (EPCs) in vitro by loading different types of micro strain stress on EPCs. We also explore the possible mechanisms of this phenomenon.
The results of flow cytometry show that appropriate micro strain stress can promote EPC proliferation. This phenomenon coincides with the upregulation of multiple genes or proteins affecting cell proliferation, including Cyclin D1, Proliferating Cell Nuclear Antigen (PCNA) and phospho-ERK1/2.
The results collectively suggest that proper mechanical stimulation can promote the proliferation of EPCs and promote angiogenesis.
本研究旨在通过丝裂原活化蛋白激酶细胞外信号调节激酶1/2（MAPK-ERK1/2）信号通路分析微应变应力对体外内皮祖细胞增殖的影响。
在本研究中，我们通过对内皮祖细胞加载不同类型的微应变应力，报道了微应变应力对体外内皮祖细胞（EPCs）增殖的影响。我们还探讨了这一现象的可能机制。
流式细胞术结果显示，适当的微应变应力可促进内皮祖细胞增殖。这一现象与影响细胞增殖的多个基因或蛋白的上调相一致，包括细胞周期蛋白D1、增殖细胞核抗原（PCNA）和磷酸化ERK1/2。
这些结果共同表明，适当的机械刺激可促进内皮祖细胞增殖并促进血管生成。
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