Zou Yajie, Zhang Meijing, Qu Jibin, Zhang Jinxia
Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Microbiol. 2018 Oct 9;9:2368. doi: 10.3389/fmicb.2018.02368. eCollection 2018.
High temperature is a key limiting factor for mycelium growth and development in . Thermotolerance includes the direct response to heat stress and the ability to recover from heat stress. To better understand the mechanism of thermotolerance in , we used morphological and physiological analysis combined with an iTRAQ-based proteomics analysis of subjected to 40°C for 48 h followed by recovery at 25°C for 3 days. High temperature increased the concentrations of thiobarbituric acid reactive substances (TBARS) indicating that the mycelium of were damaged by heat stress. However, these physiological changes rapidly returned to control levels during the subsequent recovery phase from heat stress. In comparison to unstressed controls, a total of 204 proteins were changed during heat stress and/or the recovery phase. Wherein, there were 47 proteins that responded to both stress and recovery conditions, whereas 84 and 73 proteins were responsive to only heat stress or recovery conditions, respectively. Furthermore, quantitative real-time PCR (qRT-PCR) confirmed differential expression of nine candidate genes revealed that some of the proteins, such as a mitogen-activated protein kinase (MAPK), phenylalanine ammonia-lyase (PAL), and heat shock protein (HSP), were also regulated by heat stress at the level of transcription. These differentially expressed proteins (DEPs) in mycelium of under heat stress were from 13 biological processes. Moreover, protein-protein interaction analysis revealed that proteins involved in carbohydrate and energy metabolism, signal transduction, and proteins metabolism could be assigned to three heat stress response networks. On the basis of these findings, we proposed that effective regulatory protein expression related to MAPK-pathway, antioxidant enzymes, HSPs, and other stress response proteins, and glycolysis play important roles in enhancing adaptation to and recovery from heat stress. Of note, this study provides useful information for understanding the thermotolerance mechanism for basidiomycetes.
高温是[某种生物]菌丝体生长和发育的关键限制因素。耐热性包括对热应激的直接反应以及从热应激中恢复的能力。为了更好地理解[某种生物]的耐热机制,我们采用形态学和生理学分析,并结合基于iTRAQ的蛋白质组学分析,对[某种生物]在40°C处理48小时后于25°C恢复3天的样本进行研究。高温增加了硫代巴比妥酸反应性物质(TBARS)的浓度,表明[某种生物]的菌丝体受到热应激的损伤。然而,在随后从热应激恢复的阶段,这些生理变化迅速恢复到对照水平。与未受应激的对照相比,在热应激和/或恢复阶段共有204种蛋白质发生了变化。其中,有47种蛋白质对应激和恢复条件均有反应,而分别有84种和73种蛋白质仅对热应激或恢复条件有反应。此外,定量实时PCR(qRT-PCR)证实了9个候选基因的差异表达,表明一些蛋白质,如丝裂原活化蛋白激酶(MAPK)、苯丙氨酸解氨酶(PAL)和热休克蛋白(HSP),在转录水平上也受到热应激的调节。热应激下[某种生物]菌丝体中这些差异表达的蛋白质(DEPs)来自13个生物学过程。此外,蛋白质-蛋白质相互作用分析表明,参与碳水化合物和能量代谢、信号转导以及蛋白质代谢的蛋白质可被归入三个热应激反应网络。基于这些发现,我们提出与MAPK途径、抗氧化酶、HSPs和其他应激反应蛋白相关的有效调节蛋白表达以及糖酵解在增强[某种生物]对热应激的适应和恢复中起重要作用。值得注意的是,本研究为理解担子菌的耐热机制提供了有用信息。