College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Haixia Institute of Science and Technology, Horticultural Plant Biology and Metabolomics Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plant Physiol. 2017 Oct;175(2):816-827. doi: 10.1104/pp.17.00814. Epub 2017 Aug 18.
Tissue organization and pattern formation within a multicellular organism rely on coordinated cell division and cell-fate determination. In animals, cell fates are mainly determined by a cell lineage-dependent mechanism, whereas in plants, positional information is thought to be the primary determinant of cell fates. However, our understanding of cell-fate regulation in plants mostly relies on the histological and anatomical studies on Arabidopsis () roots, which contain a single layer of each cell type in nonvascular tissues. Here, we investigate the dynamic cell-fate acquisition in modified Arabidopsis roots with additional cell layers that are artificially generated by the misexpression of (). We found that cell-fate determination in Arabidopsis roots is a dimorphic cascade with lineage inheritance dominant in the early stage of pattern formation. The inherited cell identity can subsequently be removed or modified by positional information. The instruction of cell-fate conversion is not a fast readout during root development. The final identity of a cell type is determined by the synergistic contribution from multiple layers of regulation, including symplastic communication across tissues. Our findings underline the collaborative inputs during cell-fate instruction.
多细胞生物体内的组织和模式形成依赖于细胞分裂和细胞命运决定的协调。在动物中，细胞命运主要由细胞谱系依赖性机制决定，而在植物中，位置信息被认为是细胞命运的主要决定因素。然而，我们对植物中细胞命运调控的理解主要依赖于对拟南芥（Arabidopsis）根的组织学和解剖学研究，拟南芥根中非脉管组织中每一种细胞类型只有一层。在这里，我们通过过表达（）来研究具有额外细胞层的改良拟南芥根中的动态细胞命运获得。我们发现，拟南芥根中的细胞命运决定是一个二态级联，在形态发生的早期阶段，谱系遗传占主导地位。随后，位置信息可以去除或改变遗传的细胞身份。细胞命运转换的指令不是根发育过程中的快速读出。细胞类型的最终身份由多层次调控的协同贡献决定，包括组织间的胞质连通性。我们的研究结果强调了细胞命运指令过程中的协作输入。
