Bauwe Hermann
Plant Physiology Department, University of Rostock, 18059, Rostock, Germany.
Methods Mol Biol. 2017;1653:31-50. doi: 10.1007/978-1-4939-7225-8_3.
The determination of enzyme activities in organ or organellar extracts is an important means of investigating metabolic networks and allows testing the success of enzyme-targeted genetic engineering. It also delivers information on intrinsic enzyme parameters such as kinetic properties or impact of effector molecules. This chapter provides protocols on how to assess activities of the enzymes of the core photorespiratory pathway, from 2-phosphoglycolate phosphatase to glycerate 3-kinase.
测定器官或细胞器提取物中的酶活性是研究代谢网络的重要手段，并且能够检验以酶为靶点的基因工程是否成功。它还能提供有关酶的内在参数信息，如动力学特性或效应分子的影响。本章提供了关于如何评估核心光呼吸途径中从2-磷酸乙醇酸磷酸酶到甘油酸3-激酶的酶活性的实验方案。
