Du Xiao, Lu Chang, He Xiao-Li, Du Guan-Hua
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
College of Traditional Chinese Medicine and Western Medicine, Anhui University of Chinese Medicine, Hefei 230038, China.
Zhongguo Zhong Yao Za Zhi. 2017 Jun;42(11):2139-2145. doi: 10.19540/j.cnki.cjcmm.2017.0095.
To observe the effect of active components group of Xiaoxuming decoction (XXMD) on brain mitochondria in cerebral ischemia/reperfusion rats during early recovery period, and study its protective mechanism for nerves in cerebral ischemia/reperfusion rats during early recovery period. Cerebral ischemia model of middle cerebral artery occlusion in rats was established by suture method, and reperfusion was conducted 2 h later. The degree of cerebral ischemia in rats was evaluated by using Zea-Longa's standard grading method, and the model rats were randomly divided into model group, Xiaoxuming decoction active components low, medium and high dose groups and positive drug Ginaton group, with sham operated rats as control group. Gradient centrifugation was used to extract the mitochondria from rat brain after 5 days of drug administration. Then the mitochondrial respiratory function was measured by Clark oxygen electrode method; mitochondrial membrane potential and the mitochondrial reactive oxygen species(ROS) level were detected by fluorescence probe methods; and the activity of mitochondrial succinodehydrogenase (SDH) and the content of ATP in the ischemic region of MCAO rats were measured by spectrophotometric method. The results showed that as compared with the model group, XXMD could significantly improve mitochondrial respiratory activity, increase the activity of SDH, reduce the level of ROS, increase mitochondrial membrane potential and obviously promote the synthesis of ATP in brain tissues. The results indicated that XXMD active components group could alleviate the energy metabolism disorders, protect brain mitochondrial damage and improve mitochondrial function in MCAO rats, which may be the mechanism of its neuroprotection activity.
观察消栓明汤有效成分组（XXMD）对脑缺血/再灌注大鼠早期恢复阶段脑线粒体的影响，探讨其对脑缺血/再灌注大鼠早期恢复阶段神经的保护机制。采用线栓法建立大鼠大脑中动脉闭塞脑缺血模型，2 h后进行再灌注。采用Zea-Longa标准评分法评估大鼠脑缺血程度，将模型大鼠随机分为模型组、消栓明汤有效成分低、中、高剂量组及阳性药物金纳多组，以假手术大鼠作为对照组。给药5 d后，采用梯度离心法提取大鼠脑组织线粒体。然后用Clark氧电极法测定线粒体呼吸功能；用荧光探针法检测线粒体膜电位及线粒体活性氧（ROS）水平；用分光光度法测定MCAO大鼠缺血区线粒体琥珀酸脱氢酶（SDH）活性及ATP含量。结果显示，与模型组比较，XXMD可显著提高线粒体呼吸活性，增加SDH活性，降低ROS水平，提高线粒体膜电位，并明显促进脑组织ATP合成。结果表明，XXMD有效成分组可减轻MCAO大鼠能量代谢紊乱，保护脑线粒体损伤，改善线粒体功能，这可能是其神经保护作用的机制。
