Si Ji-Ping, Wang Qi, Liu Zhong-Jian, Liu Jing-Jing, Luo Yi-Bo
Dendrobium State Forestry Engineering Research Center,State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Linan 311300, China.
Shenzhen Key Laboratory for Orchid Conservation and Utilization,the National Orchid Conservation Center of China and the Orchid Conservation and Research Center of Shenzhen,Shenzhen 518114,China.
Zhongguo Zhong Yao Za Zhi. 2017 Jun;42(12):2223-2227. doi: 10.19540/j.cnki.cjcmm.2017.0102.
In view of the significant difficulties of propagation, planting and simple product in Dendrobium catenatum(D. officinale)industry development, a series of research were carried out. Genome study showed that D. catenatum is a specie of diploid with 38 chromosomes and 28 910 protein-coding genes. It was identified that specific genes accumulated in different organs at the transcriptome level. We got an insight into the gene regulation mechanism of the loss of the endospermous seed, the wide ecological adaptability and the synthesis of polysaccharides, which provided a theoretical basis for genetic engineering breeding and development and utilization of active pharmaceutical ingredients. The rapid propagation system was established for applying to industrialized production by overcoming breeding problems on seed setting and sprouting, which laid a foundation for artificial cultivation of D. catenatum. And in order to give a clear explanation of genetic variation of important economic traits, we built up the breeding system. Since special varieties of D. catenatum were bred, it helped solve the problem of trait segregation of seedling progeny and support the improvement of D. catenatum industry. The regulation of dynamic variation of target compounds, together with the mechanism of nutrient uptake, was revealed. The breakthrough of key technologies including culture substrates, light regulation and precisely collection was carried out. Several cultivation modes like facility cultivation, original ecological cultivation, cliff epiphytic cultivation, stereoscopic cultivation and potting cultivation were set up. Above all, the goal of cultivating D. catenatum as well as producing good D. catenatum will be achieved.
鉴于铁皮石斛产业发展中繁殖、种植及初级产品存在的重大困难，开展了一系列研究。基因组研究表明，铁皮石斛是二倍体物种，有38条染色体和28910个蛋白质编码基因。在转录组水平上鉴定出不同器官中积累的特定基因。我们深入了解了无胚乳种子丧失、广泛生态适应性和多糖合成的基因调控机制，为基因工程育种及活性药用成分的开发利用提供了理论依据。通过克服结实和发芽方面的育种问题，建立了适用于工业化生产的快速繁殖体系，为铁皮石斛的人工栽培奠定了基础。为了清晰阐释重要经济性状的遗传变异，我们构建了育种体系。自培育出铁皮石斛特殊品种后，有助于解决幼苗子代性状分离问题，推动铁皮石斛产业升级。揭示了目标化合物动态变化规律及养分吸收机制。在栽培基质、光照调控和精准采收等关键技术方面取得突破。建立了设施栽培、原生态栽培、悬崖附生栽培、立体栽培和盆栽等多种栽培模式。总之，将实现种植优质铁皮石斛及生产优质铁皮石斛的目标。
