Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
Bioresour Technol. 2017 Nov;244(Pt 1):717-725. doi: 10.1016/j.biortech.2017.08.032. Epub 2017 Aug 9.
In this study, bamboo (Phyllostachys pubescens) was successfully deconstructed using an integrated process (autohydrolysis and subsequent delignification). Xylooligosaccharides, high-purity lignin, and digestible substrates for producing glucose can be consecutively collected during the integrated process. The structural change and fate of lignin during autohydrolysis process was deeply investigated. Additionally, the structural characteristics and active functional groups of the lignin fractions obtained by these delignification processes were thoroughly investigated by NMR (2D-HSQC and P NMR) and GPC techniques. The chemical compositions (S, G, and H) and major linkages (β-O-4, β-β, β-5, etc.) were thoroughly assigned and the frequencies of the major lignin linkages were quantitatively compared. Considering the structural characteristics and molecular weights of the lignin as well as enzymatic saccharification ratio of the substrate, the combination of autohydrolysis and organic base-catalyzed ethanol pretreatment was deemed as a promising biorefinery mode in the future based on bamboo feedstock.
在这项研究中，成功地使用集成工艺（自水解和随后的脱木质素）对竹子（毛竹）进行了解构。在集成工艺过程中，可以连续收集木低聚糖、高纯度木质素和可用于生产葡萄糖的可消化底物。深入研究了木质素在自水解过程中的结构变化和命运。此外，通过 NMR（2D-HSQC 和 P NMR）和 GPC 技术彻底研究了这些脱木质素过程中获得的木质素级分的结构特征和活性官能团。彻底分配了化学组成（S、G 和 H）和主要键合（β-O-4、β-β、β-5 等），并定量比较了主要木质素键的频率。考虑到木质素的结构特征和分子量以及底物的酶糖化率，基于竹原料，自水解和有机碱催化的乙醇预处理的结合被认为是未来有前途的生物炼制模式。
