Trzebuniak Kamil F, Mysliwa-Kurdziel Beata
Department of Plant Physiology and Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland.
Acta Biochim Pol. 2017;64(3):431-436. doi: 10.18388/abp.2017_2207. Epub 2017 Aug 19.
The aim of this analytical study was to develop and validate an easy-to-use method for measuring the actual level of norflurazon that accumulates in leaves. We amended the QuEChERS method, i.e. Quick, Easy, Cheap, Effective, Rugged, and Safe, which is widely used for pesticide and herbicide analysis in food, and usually combined with HPLC-MS detection. We adapted this method for the detection of norflurazon in leaves or leaf fragments and proposed a useful modification using of HPLC-UV detection. Reproducible retention times of 3.11±0.04 min, precision (RSD<8.0%), LOQ=315 ng∙mL and linearity (R=0.99874) were achieved.
本分析研究的目的是开发并验证一种易于使用的方法，用于测量叶片中积累的去草净实际含量。我们改进了QuEChERS方法，即快速、简便、廉价、高效、耐用且安全的方法，该方法广泛用于食品中农药和除草剂的分析，通常与高效液相色谱-质谱检测联用。我们将此方法适用于叶片或叶片碎片中去草净的检测，并提出了一种使用高效液相色谱-紫外检测的有用改进方法。实现了3.11±0.04分钟的可重现保留时间、精密度（相对标准偏差<8.0%）、定量限=315 ng∙mL以及线性（R=0.99874）。
