Marangoni Laura Fernandes de Barros, Marques Joseane Aparecida, Duarte Gustavo Adolpho Santos, Pereira Cristiano Macedo, Calderon Emiliano Nicolas, Castro Clovis Barreira E, Bianchini Adalto
Programa de Pós-Graduação em Oceanografia Biológica, Instituto de Oceanografia, Universidade Federal do Rio Grande, Av. Itália, km 8, Rio Grande, RS 96203-900, Brazil.
Programa de Pós-graduação em Zoologia, Museu Nacional, Universidade Federal do Rio de Janeiro, Parque Quinta da Boa Vista, São Cristovão, Rio de Janeiro, RJ 20940-040, Brazil.
Mar Environ Res. 2017 Sep;130:248-257. doi: 10.1016/j.marenvres.2017.08.002. Epub 2017 Aug 10.
Seawater contamination with metals, such as copper (Cu), is a notable local impact threatening coral reefs. Cu effects on biomarkers associated with photosynthesis, oxidative status and calcification were evaluated in the Brazilian coral Mussismilia harttii using a marine mesocosm facility. Polyps were kept under control conditions (1.9 μg L Cu) or exposed to dissolved Cu (3.0, 4.8, and 6.7 μg L) for 12 days. Photochemical efficiency of the photosystem II of symbiotic algae (zooxanthellae) was measured and polyps were analyzed for antioxidant capacity, lipid peroxidation, DNA damage, and carbonic anhydrase Ca-ATPase, Mg-ATPase and (Ca,Mg)-ATPase activities after 12 days. Results highlighted the effects of Cu exposure, leading corals to an oxidative stress condition [increased total antioxidant capacity (TAC) and DNA damage] and a possible reduced calcification ability [decreased (Ca,Mg)-ATPase activity]. Therefore, biomarkers associated with oxidative status (TAC and DNA damage) and calcification [(Ca, Mg)-ATPase] are indicated as good predictors of corals health.
海水中的金属污染，如铜（Cu）污染，是威胁珊瑚礁的一个显著的局部影响因素。利用海洋中宇宙设施，对巴西珊瑚哈氏缪氏珊瑚（Mussismilia harttii）中与光合作用、氧化状态和钙化相关的生物标志物的铜影响进行了评估。将珊瑚虫置于对照条件下（1.9微克/升铜）或暴露于溶解铜（3.0、4.8和6.7微克/升）中12天。测量共生藻类（虫黄藻）光系统II的光化学效率，并在12天后分析珊瑚虫的抗氧化能力、脂质过氧化、DNA损伤以及碳酸酐酶、钙-ATP酶、镁-ATP酶和（钙，镁）-ATP酶活性。结果突出了铜暴露的影响，导致珊瑚处于氧化应激状态[总抗氧化能力（TAC）增加和DNA损伤]以及可能的钙化能力降低[（钙，镁）-ATP酶活性降低]。因此，与氧化状态（TAC和DNA损伤）和钙化[（钙，镁）-ATP酶]相关的生物标志物被认为是珊瑚健康的良好预测指标。
