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用于不同病毒感染的成簇规律间隔短回文重复序列-Cas系统的治疗策略

Therapeutic Strategies of Clustered Regularly Interspaced Palindromic Repeats-Cas Systems for Different Viral Infections.

作者信息

Wahid Braira, Usman Sana, Ali Amjad, Saleem Komal, Rafique Shazia, Naz Zara, Ahsan Ashfaq Hafiz, Idrees Muhammad

机构信息

1 Centre for Applied Molecular Biology, University of the Punjab , Lahore, Pakistan .

2 Center of Excellence in Molecular Biology, University of the Punjab , Lahore, Pakistan .

出版信息

Viral Immunol. 2017 Oct;30(8):552-559. doi: 10.1089/vim.2017.0055. Epub 2017 Aug 21.

Abstract

The clustered regularly interspaced palindromic repeats (CRISPR)/Cas systems have rapidly transitioned from intriguing prokaryotic defense systems to an efficient therapeutic tool. This cutting-edge technology is currently revolutionizing to combat hostile viruses because of its reproducibility, high potency, ease of use, limited off-target activity, and development of quick immune response against viruses. CRISPR gene editing technology eliminates the virus by breaking the DNA that ultimately halts viral replication. This review summarizes the advancements that have been made in the use of CRISPR-Cas technology in viral therapeutics.

摘要

成簇规律间隔短回文重复序列(CRISPR)/Cas系统已迅速从有趣的原核生物防御系统转变为一种高效的治疗工具。这项前沿技术目前正在进行变革以对抗有害病毒,因为它具有可重复性、高效性、易用性、脱靶活性有限以及能快速产生针对病毒的免疫反应。CRISPR基因编辑技术通过切断最终阻止病毒复制的DNA来消除病毒。本综述总结了CRISPR-Cas技术在病毒治疗应用方面所取得的进展。

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