School of Chemical and Biomedical Engineering, Nanyang Technological University, 637457 Singapore, Singapore.
Department of Biomedical Engineering, National University of Singapore, 117583 Singapore, Singapore.
Biosens Bioelectron. 2018 Jan 15;99:578-585. doi: 10.1016/j.bios.2017.08.012. Epub 2017 Aug 8.
An interferometric fiber sensor for detection of hexa-histidine tagged microcin (His-MccS) is reported and experimentally demonstrated. This intermodal fiber sensor is implemented by a no-core fiber (NCF) functionalized with chitosan (CS)-nickel (Ni) film for direct detection of small peptide: microcin. The fiber intermodal sensor relies on the refractive index modulations due to selective adsorption event at the chitosan (CS)-nickel (Ni) film. Owing to the strong affinity between Ni ions and histidine, the immobilized Ni ions in the chitosan film were utilized as binding agents for the direct detection of hexa-histidine tagged microcin. A comparative study in relation to different target size was conducted: full proteins trypsin, bovine serum albumin (BSA) and human serum albumin (HSA), with high histidine content on their surface and His-MccS (peptide, 11.6kDa), have been employed for sensor evaluation. Results have shown selectivity for His-MccS relative to trypsin, BSA and HSA. The most telling contribution of this study is the fast detection of small biomolecule His-MccS compared to standard detection procedures like SDS-PAGE and western blot. The proposed sensor exhibits His-MccS detection sensitivity of 0.0308nm/(ng/ml) in the range of (0-78) ng/ml with concentration detection limit of 0.8368ng/ml.
一种用于检测六组氨酸标记的微菌素（His-MccS）的干涉型光纤传感器已被报道并进行了实验验证。这种多模光纤传感器是通过在无芯光纤（NCF）上功能化壳聚糖（CS）-镍（Ni）薄膜来实现的，用于直接检测小肽：微菌素。光纤多模传感器依赖于壳聚糖（CS）-镍（Ni）薄膜上选择性吸附事件引起的折射率调制。由于 Ni 离子与组氨酸之间的强亲和力，壳聚糖薄膜中固定的 Ni 离子被用作直接检测六组氨酸标记的微菌素的结合剂。进行了与不同目标大小相关的比较研究：表面具有高组氨酸含量的全蛋白胰蛋白酶、牛血清白蛋白（BSA）和人血清白蛋白（HSA）以及 His-MccS（肽，11.6kDa）已被用于传感器评估。结果表明，相对于胰蛋白酶、BSA 和 HSA，该传感器对 His-MccS 具有选择性。这项研究最有意义的贡献是与 SDS-PAGE 和 Western blot 等标准检测程序相比，能够快速检测小分子生物标志物 His-MccS。所提出的传感器在（0-78）ng/ml 的范围内对 His-MccS 的检测灵敏度为 0.0308nm/（ng/ml），浓度检测限为 0.8368ng/ml。
