Iwasaki K, Mano K, Ishihara M, Yugari Y, Matsuzawa T
Biochem Int. 1987 Jan;14(1):129-34.
Using rat heart perfusion, we found that an anabolic state can be induced with a medium which includes glucose, carnitine, branched-chain amino acids and arginine after arginine intubation at a dose of 250 mg/kg body weight. It showed diminished levels of glutamine, glutamate, branched-chain oxoacids and phenylalanine (a marker of heart protein metabolism) release, reflecting anabolic changes occurring in the myocardium. While ornithine intubation caused a catabolic state in which the release of alanine and glutamate was increased but phenylalanine release was unchanged. This anabolic state may be a useful model providing for myocardial protection.
通过大鼠心脏灌注实验，我们发现，在以250毫克/千克体重的剂量插管注入精氨酸后，使用包含葡萄糖、肉碱、支链氨基酸和精氨酸的培养基可诱导合成代谢状态。结果显示，谷氨酰胺、谷氨酸、支链酮酸和苯丙氨酸（心脏蛋白质代谢的一个标志物）的释放水平降低，这反映了心肌中发生的合成代谢变化。而插管注入鸟氨酸则导致分解代谢状态，其中丙氨酸和谷氨酸的释放增加，但苯丙氨酸的释放没有变化。这种合成代谢状态可能是一种有用的心肌保护模型。
