First Affiliated Hospital of Dalian Medical University, Dalian, China.
Sci Rep. 2017 Aug 21;7(1):8356. doi: 10.1038/s41598-017-08548-z.
Genome-wide association studies identified that the common T of rs12143842 in NOS1AP is associated with a QT/QTc interval in European populations. In this study, we test the association between the variation rs12143842 in NOS1AP and idiopathic ventricular tachycardia (IVT). A case-control association study examining rs12143842 was performed in two independent cohorts. The Northern cohort enrolled 277 IVT patients and 728 controls from a Chinese Gene ID population. The Central cohort enrolled 301 IVT patients and 803 matched controls. Genotyping was performed using high-resolution melt analysis. The minor T allele of the rs12143842 SNP was significantly associated with decreased IVT risk in the Northern cohort (adjusted P = 0.024, OR 0.71(0.520.96)), and this association was replicated in an independent Central Gene ID cohort (adjusted P = 0.029, OR 0.78 (0.620.97)). The association was more significant in the combined population (adjusted P = 0.001, OR 0.76 (0.64~0.90)). The P values for the genotypic association were significant for the dominant (P < 0.001) and additive (P = 0.001) models. The minor T allele for the SNP rs12143842 in NOS1AP is significantly associated with IVT. NOS1AP might be a novel gene affecting IVT, and further functional studies should be performed.
全基因组关联研究表明，NOS1AP 基因上 rs12143842 的常见 T 等位基因与欧洲人群的 QT/QTc 间期有关。在这项研究中，我们检验了 NOS1AP 基因中的变异 rs12143842 与特发性室性心动过速（IVT）之间的关联。我们在两个独立的队列中进行了 NOS1AP 基因 rs12143842 变异的病例对照关联研究。北方队列纳入了来自中国基因 ID 人群的 277 例 IVT 患者和 728 例对照。中央队列纳入了 301 例 IVT 患者和 803 例匹配对照。采用高分辨率熔解分析进行基因分型。rs12143842 单核苷酸多态性的次要 T 等位基因与北方队列中 IVT 风险降低显著相关（调整后的 P=0.024，OR 0.71（0.520.96）），这种关联在独立的中央基因 ID 队列中得到了复制（调整后的 P=0.029，OR 0.78（0.620.97））。在合并人群中，这种关联更为显著（调整后的 P=0.001，OR 0.76（0.64~0.90））。对于显性（P<0.001）和加性（P=0.001）模型，基因型关联的 P 值均具有统计学意义。NOS1AP 基因 rs12143842 中的 SNP 次要 T 等位基因与 IVT 显著相关。NOS1AP 可能是一个影响 IVT 的新基因，应该进行进一步的功能研究。
