Ketis N V
Biochim Biophys Acta. 1987 May 19;924(2):303-11. doi: 10.1016/0304-4165(87)90027-4.
Two-dimensional peptide map analysis was used to determine the structural homology among the '100 kDa'-group of polypeptides. There are at least six distinct polypeptides whose apparent molecular weights are 116, 113, 111, 108, 105 and 100 kDa. The molar ratio of the '100 kDa'-group of polypeptides to three clathrin monomers (equivalent to one triskelion) is 1.2:1. There are three families of polypeptides in the '100 kDa'-group as determined by two-dimensional peptide map analysis. They are 116 and 113 kDa polypeptides, 111, 108, and 105 kDa polypeptides and 100 kDa polypeptide. However, all six polypeptides apparently show a series of homologous peptides. It is suggested that the 100-116 kDa polypeptides may bind to triskelions at the area of homology that is found in the 100-116 kDa polypeptides.
二维肽图分析用于确定“100 kDa”多肽组之间的结构同源性。至少有六种不同的多肽，其表观分子量分别为116、113、111、108、105和100 kDa。“100 kDa”多肽组与三个网格蛋白单体（相当于一个三脚蛋白复合体）的摩尔比为1.2:1。通过二维肽图分析确定，“100 kDa”多肽组中有三个多肽家族。它们是116和113 kDa多肽、111、108和105 kDa多肽以及100 kDa多肽。然而，所有六种多肽显然都显示出一系列同源肽段。有人提出，100 - 116 kDa多肽可能在100 - 116 kDa多肽中发现的同源区域与三脚蛋白复合体结合。
