基于纸张的细胞外囊泡分离方法

Paper-Based for Isolation of Extracellular Vesicles.

作者信息

Hsiao Yi-Hsing, Chen Chihchen

机构信息

Institute of Nanoengineering and Microsystems, National Tsing Hua University, No.101, Section 2, Guangfu Rd., East Dist, Hsinchu, 30013, Taiwan.

Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli, 35053, Taiwan.

出版信息

Methods Mol Biol. 2017;1660:43-54. doi: 10.1007/978-1-4939-7253-1_5.

DOI:10.1007/978-1-4939-7253-1_5

PMID:28828647

Abstract

Paper-based devices chemically functionalized with capturing molecules enable the isolation and characterization of extracellular vesicles (EVs) from samples of limited amount. Here, we describe the isolation of EV subpopulations from human serum samples. The morphology, content, and amount of captured EVs can be assessed using scanning electron microscopy (SEM ), transcriptome analysis, and paper-based enzyme-linked immunosorbent assays (pELISA), respectively. A colorimetric readout can be detected from 10 μL serum within 10 min.

摘要

用捕获分子进行化学功能化的纸质设备能够从少量样品中分离和表征细胞外囊泡（EVs）。在此，我们描述了从人血清样本中分离EV亚群的方法。捕获的EVs的形态、内容物和数量可分别使用扫描电子显微镜（SEM）、转录组分析和基于纸的酶联免疫吸附测定（pELISA）进行评估。10分钟内可从10μL血清中检测到比色读数。