肝病患者血清γ-谷氨酰转移酶的凝集素亲和层析法

Lectin-affinity chromatography of serum gamma-glutamyltransferase in liver disease.

作者信息

Delanghe J R, De Buyzere M L, De Scheerder I K, Claeys L R, Wieme R J

出版信息

Clin Chim Acta. 1987 Feb 15;162(3):311-8. doi: 10.1016/0009-8981(87)90050-7.

Abstract

The variation of the carbohydrate chain of gamma-glutamyltransferase was studied in 45 liver patients by means of lectin affinity chromatography. Five lectins were used: concanavalin A, Ricinus communis I and II, Maclura pomifera and Ulex europaeus agglutinin. The binding towards Con A was shown to be independent from the binding towards the other lectins. Parallel variations of binding results against the galactose- and fucose-recognizing lectins were obtained. In liver steatosis, the binding results were comparable to those obtained in normal patients. Cirrhosis and metastasis patients showed a decreased binding towards Con A, while the binding against the various galactose- and fucose-recognizing lectins was increased. After neuraminidase treatment, an increased affinity towards all lectins was observed. However, differences in RCA I and RCA II binding between patients and controls still persisted. Besides sialic acid, also galactose and fucose residues contribute to serum gamma-glutamyltransferase heterogeneity.

摘要

通过凝集素亲和色谱法研究了45例肝病患者γ-谷氨酰转移酶碳水化合物链的变化。使用了五种凝集素：伴刀豆球蛋白A、蓖麻凝集素I和II、桑科榕属植物凝集素和荆豆凝集素。结果表明，与伴刀豆球蛋白A的结合与与其他凝集素的结合无关。获得了针对识别半乳糖和岩藻糖的凝集素的结合结果的平行变化。在肝脂肪变性中，结合结果与正常患者相当。肝硬化和转移患者对伴刀豆球蛋白A的结合减少，而对各种识别半乳糖和岩藻糖的凝集素的结合增加。神经氨酸酶处理后，观察到对所有凝集素的亲和力增加。然而，患者与对照组之间在蓖麻凝集素I和蓖麻凝集素II结合方面的差异仍然存在。除了唾液酸外，半乳糖和岩藻糖残基也有助于血清γ-谷氨酰转移酶的异质性。