Itokazu M, Bradley J, Francis M J, Duthie R B
Nuffield Department of Orthopaedic Surgery, University of Oxford, Nuffield Orthopaedic Center, Headington, England.
Clin Orthop Relat Res. 1988 May(230):284-8.
Fluorescein isothiocyanate-conjugated lectins include: concanavalin A (Con-A), Dolichos biflorus agglutinin (DBA), Griffonia simplicifolia-I (GS-I), Griffonia simplicifolia-II (GS-II), Arachis hypogaea agglutinin (PNA), Maclura pomifera agglutinin (MPA), Ricinus communis agglutinin-I (RCA-I), Glycine max agglutinin (SBA), Ulex europaeus agglutinin-I (UEA-I), and wheat germ agglutinin (WGA). These lectins were used to histochemically demonstrate lectin bindings on hemophilic synovial membrane. GS-I (galactose and galactosamine specific) and SBA (galactosamine specific) was shown to bind strongly in the cytoplasm of the lining cells. Lymphocytes and mast cells were largely bound by Con-A (mannose and glucose specific) and GS-II (glucosamine specific) positive. UEA-1 (fucose specific) was shown to bind specifically to synovial vascular endothelial cells. Lectin histochemistry is a useful method for classification of the cells on the synovial membrane.
刀豆球蛋白A（Con-A）、双花扁豆凝集素（DBA）、西非单叶豆凝集素-I（GS-I）、西非单叶豆凝集素-II（GS-II）、花生凝集素（PNA）、桑科柘树凝集素（MPA）、蓖麻凝集素-I（RCA-I）、大豆凝集素（SBA）、荆豆凝集素-I（UEA-I）和麦胚凝集素（WGA）。这些凝集素用于组织化学显示凝集素在血友病性滑膜上的结合。GS-I（对半乳糖和氨基半乳糖具有特异性）和SBA（对氨基半乳糖具有特异性）在衬里细胞的细胞质中显示出强烈结合。淋巴细胞和肥大细胞大多被Con-A（对甘露糖和葡萄糖具有特异性）和GS-II（对氨基葡萄糖具有特异性）阳性结合。UEA-1（对岩藻糖具有特异性）显示出特异性结合滑膜血管内皮细胞。凝集素组织化学是滑膜细胞分类的一种有用方法。
