Ishaan Dilshi, Parthasarathy Harinath, Ponnaiyan Deepa, Tadepalli Anupama
Department of Periodontics, SRM Dental College , Ramapuram, Chennai, Tamil Nadu, India .
Genet Test Mol Biomarkers. 2017 Sep;21(9):560-564. doi: 10.1089/gtmb.2017.0029. Epub 2017 Aug 22.
The genetic variation in the CD14 rs2569190 promoter region contributes to the susceptibility of developing periodontitis. Since, CD14 gene polymorphism studies among various ethnic populations have produced conflicting results, the present study was designed to unearth the association between the CD14 rs2569190 gene polymorphism and generalized chronic periodontitis (GCP) in a South Indian population of Tamil ethnicity.
Polymorphisms in the CD14 rs2569190 promoter region were analyzed in 96 subjects who were periodontally healthy and 96 patients with GCP. DNA extracted from peripheral venous blood was genotyped by polymerase chain reaction-restriction fragment length polymorphism analysis. One-way analysis of variance, Chi-square testing, and logistic regression analysis were used to test association between GCP and the CD14 rs2569190 single nucleotide polymorphism (SNP).
The distribution of the CD14 rs2569190 genotypes and allele frequencies showed significant differences between the test and control groups. Prevalence of the polymorphic TT genotype and T allele of the CD14 rs2569190 were significantly increased in GCP patients when compared with healthy controls.
The results of the present study suggest that the T allele and the TT genotype of the rs2569190 SNP in the promoter region of the CD14 gene are associated with GCP in a South Indian population of Tamil ethnicity.
CD14基因rs2569190启动子区域的基因变异与患牙周炎的易感性有关。由于不同种族人群中CD14基因多态性研究结果相互矛盾，本研究旨在揭示泰米尔族裔的南印度人群中CD14 rs2569190基因多态性与广泛性慢性牙周炎（GCP）之间的关联。
对96名牙周健康受试者和96名GCP患者的CD14 rs2569190启动子区域多态性进行分析。通过聚合酶链反应-限制性片段长度多态性分析对从外周静脉血中提取的DNA进行基因分型。采用单因素方差分析、卡方检验和逻辑回归分析来检验GCP与CD14 rs2569190单核苷酸多态性（SNP）之间的关联。
CD14 rs2569190基因型和等位基因频率分布在试验组和对照组之间存在显著差异。与健康对照组相比，GCP患者中CD14 rs2569190多态性TT基因型和T等位基因的患病率显著增加。
本研究结果表明，CD14基因启动子区域rs2569190 SNP的T等位基因和TT基因型与泰米尔族裔的南印度人群中的GCP有关。
