Department of Obstetrics, Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China.
Eur Rev Med Pharmacol Sci. 2017 Aug;21(15):3504-3509.
Paclitaxel is one of the common anticancer drugs in the treatment of cervical cancer, while the mechanism of restraining and killing cancer cells is still unclear. This study aimed to investigate the molecular mechanism of paclitaxel in regulating proliferation and apoptosis of cervical cancer Hela cells.
Paclitaxel at 2 μmol/L was used to treat Hela cells for 48 h. MTT assay and flow cytometry were applied to test Hela cells proliferation and apoptosis respectively. Western blot was adopted to determine the expression of survivin. SiRNA was performed to suppress survivin protein expression in Hela cells.
Paclitaxel restrained Hela cells growth and induced apoptosis. Also, paclitaxel treatment significantly reduced survivin protein expression in Hela cells. Moreover, survivin siRNA transfection further promoted Hela cells apoptosis after intervention by 2 μmol/L paclitaxel.
Down-regulation of survivin promoted paclitaxel-induced apoptosis of cervical cancer Hela cells.
紫杉醇是治疗宫颈癌的常用抗癌药物之一，但其抑制和杀伤癌细胞的机制尚不清楚。本研究旨在探讨紫杉醇调节宫颈癌 Hela 细胞增殖和凋亡的分子机制。
用 2μmol/L 的紫杉醇处理 Hela 细胞 48h。MTT 法和流式细胞术分别检测 Hela 细胞的增殖和凋亡。Western blot 检测生存素的表达。用 siRNA 抑制 Hela 细胞中生存素蛋白的表达。
紫杉醇抑制 Hela 细胞生长并诱导其凋亡。紫杉醇处理还显著降低了 Hela 细胞中生存素蛋白的表达。此外，用 2μmol/L 紫杉醇干预后，生存素 siRNA 转染进一步促进了 Hela 细胞的凋亡。
下调生存素促进了紫杉醇诱导的宫颈癌 Hela 细胞凋亡。
