Bowness J M, Henteleff H, Dolynchuk K N
Connect Tissue Res. 1987;16(1):57-70. doi: 10.3109/03008208709001994.
To study the glycoproteins and transglutaminase substrates involved in healing, wounds were made in the skin of anesthetized rats with a biopsy punch. Explants made 1-5 days later were incubated with [3H]-labelled putrescine, fucose or proline. As compared with unwounded skin there was an increased incorporation of label which was greatest at 3 days. Incubation for various times suggests that the incorporation of fucose and proline is dependent on protein synthesis, whereas putrescine is incorporated into preformed proteins. Putrescine and fucose label polypeptides with an Mr of about 45,000 before and 14,000 after reduction. These correspond in size with the aminopropeptide of type III collagen. Other labelled material of higher molecular weight is partly degraded to similar polypeptides on collagenase digestion. Much of the [3H]putrescine in the polypeptides is in the form of gamma-glutamyl putrescine. It is hypothesized that isopeptide linkage of the aminopropeptide III occurs in wound healing.
为研究参与愈合过程的糖蛋白和转谷氨酰胺酶底物，用活检打孔器在麻醉大鼠的皮肤上制造伤口。1至5天后制备的组织外植体与[3H]标记的腐胺、岩藻糖或脯氨酸一起孵育。与未受伤的皮肤相比，标记物的掺入增加，在第3天达到最大。不同时间的孵育表明，岩藻糖和脯氨酸的掺入依赖于蛋白质合成，而腐胺则掺入预先形成的蛋白质中。还原前，腐胺和岩藻糖标记的多肽分子量约为45,000，还原后为14,000。这些大小与III型胶原的氨基端前肽相符。其他较高分子量的标记物质在胶原酶消化后部分降解为类似的多肽。多肽中的大部分[3H]腐胺以γ-谷氨酰腐胺的形式存在。据推测，氨基端前肽III的异肽键在伤口愈合中发生。
