Department of Urology, the Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou City, Guangdong Province, China.
Eur Rev Med Pharmacol Sci. 2017 Aug;21(15):3347-3352.
To investigate the clinical significance of the expression of PSMA (prostate specific membrane antigen), TERT (telomerase reverse transcriptase), and PDEF (prostate derived Ets factor) in malignant tumors of the prostate.
The study was conducted with paraffin slices from 33 specimens of malignant tumors of the prostate and 17 of normal tissue. We found high levels of PSMA, TERT, and PDEF protein by Western blot and immunofluorescence in the malignant tumor of the prostate. We also detected upregulation of PSMA, TERT, and PDEF mRNA in the malignant tumor of the prostate, suggesting complex regulation of these three genes in prostate cancer.
Variance analysis showed statistically significant differences comparing the expression of PSMA, TERT, and PDEF in the malignant tumor of the prostate and normal tissues. The high expression of PSMA, TERT, and PDEF in the malignant tumor of the prostate suggests the important roles of these three factors in the occurrence and development of the malignant tumors of the prostate.
PSMA, TERT, and PDEF may serve as a reference for clinical diagnosis and as potential targets for the malignant tumor of the prostate therapeutics.
探讨 PSMA（前列腺特异膜抗原）、TERT（端粒酶逆转录酶）和 PDEF（前列腺衍生 Ets 因子）在前列腺恶性肿瘤中的表达的临床意义。
本研究采用 33 例前列腺恶性肿瘤标本和 17 例正常组织的石蜡切片进行研究。我们通过 Western blot 和免疫荧光法发现，前列腺恶性肿瘤中 PSMA、TERT 和 PDEF 蛋白水平升高。我们还检测到前列腺恶性肿瘤中 PSMA、TERT 和 PDEFmRNA 的上调，提示这三个基因在前列腺癌中存在复杂的调控。
方差分析显示，前列腺恶性肿瘤与正常组织中 PSMA、TERT 和 PDEF 的表达存在统计学差异。前列腺恶性肿瘤中 PSMA、TERT 和 PDEF 的高表达提示这三个因素在前列腺恶性肿瘤的发生发展中具有重要作用。
PSMA、TERT 和 PDEF 可作为临床诊断的参考指标，并可能成为前列腺恶性肿瘤治疗的潜在靶点。
