Department of Chemistry, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, United States.
Department of Chemistry, Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104, United States.
Biosens Bioelectron. 2018 Jan 15;99:593-602. doi: 10.1016/j.bios.2017.08.032. Epub 2017 Aug 15.
Cell adhesion is an essential aspect of cellular behavior. Finding innovative methods to probe the adhesion of cells in their native state can greatly advance the understanding of control and regulation of cellular behavior and their impact on human health. The quartz crystal microbalance (QCM) is a label-free, biosensing system that has, in the past fifty years, evolved from a simple acoustic based mass sensor to a powerful bioanalytical tool. Its unique capability of monitoring the cell-substrate interaction non-invasively in real time has led to the emergence of its applications in areas that are relevant to fundamental cell biology and medical research. This review is intended to provide readers an overview of the use of the QCM for examination of cell-substrate adhesion. It also describes how this innovative approach can be extended to the study of other aspects of cellular behavior, such as cell morphology, cell mechanics, cell motility, cell signaling, all of which can potentially be applied to medical diagnosis and/or pharmaceutical development. In this review a major emphasis is placed on informing readers about some of the most important practical aspects of the QCM-based cell study including data acquisition and analysis, the substrate surface manipulation, and cell manipulation.
细胞黏附是细胞行为的一个基本方面。寻找创新的方法来探测细胞在其自然状态下的黏附，可以极大地促进对细胞行为的控制和调节及其对人类健康的影响的理解。石英晶体微天平（QCM）是一种无标记的生物传感系统，在过去的五十年中，它已经从一个简单的基于声学的质量传感器发展成为一种强大的生物分析工具。它能够非侵入性地实时监测细胞-底物相互作用的独特能力，使其在与基础细胞生物学和医学研究相关的领域中的应用得到了发展。这篇综述旨在为读者提供对 QCM 用于研究细胞-底物黏附的概述。它还描述了如何将这种创新方法扩展到研究细胞行为的其他方面，如细胞形态、细胞力学、细胞迁移、细胞信号转导等，所有这些都有可能应用于医学诊断和/或药物开发。在这篇综述中，重点介绍了一些关于基于 QCM 的细胞研究的最重要的实际方面，包括数据采集和分析、基底表面处理和细胞操作。
