Department of Life Science, National Taiwan Normal University, Taipei, Taiwan.
Center for General Education, National Quemoy University, Quemoy, Taiwan.
Sci Rep. 2017 Aug 22;7(1):9096. doi: 10.1038/s41598-017-08093-9.
This study was aimed to evaluate the synaptic plasticity in projections from the dorsal lateral region (Dl) to the bilateral dorsal medial region (Dm) of the zebrafish telencephalon. The results showed that unilateral electrical stimulation of the Dl evokes a negative field potential (FP) in both the contralateral and ipsilateral side of the Dm. We tested synaptic plasticity, including high-frequency stimulation-induced LTP (HFS-LTP) and low-frequency stimulation-induced LTD (LFS-LTD). We demonstrated that HFS-induced bilateral LTP is NMDAR-dependent by the application of an NMDAR antagonist, DL-AP5 (30 μM, suprafused for 10 min), which blocked the HFS-induced LTP in both the contralateral and ipsilateral Dm. In addition, LTP was restored after DL-AP5 was washed out by continuous aCSF suprafusion. These results suggested that the potentiation is NMDAR-dependent. Either LFS (1 Hz for 20 min) or applying the mGluR agonist, DHPG (40 μM, suprafused for 10 min) successfully induced bilateral LTD for at least 1 h. Furthermore, both the contralateral fEPSP and LTP vanished after ablation of the anterior commissure. In conclusion, the results of the present study suggested that the projection between the Dl and contralateral Dm in the telencephalon of zebrafish is via the anterior commissure and possesses synaptic plasticity.
本研究旨在评估斑马鱼端脑背外侧区（Dl）至双侧背内侧区（Dm）投射中的突触可塑性。结果表明，单侧 Dl 的电刺激在双侧 Dm 的对侧和同侧均引起负场电位（FP）。我们测试了突触可塑性，包括高频刺激诱导的长时程增强（HFS-LTP）和低频刺激诱导的 LTD（LFS-LTD）。我们证明，通过应用 NMDAR 拮抗剂 DL-AP5（30μM，超灌注 10min），HFS 诱导的双侧 LTP 是 NMDAR 依赖性的，DL-AP5 阻断了双侧 Dm 中的 HFS 诱导的 LTP。此外，用连续 aCSF 超灌注冲洗 DL-AP5 后，LTP 得到恢复。这些结果表明，增强是 NMDAR 依赖性的。无论是 LFS（1Hz 持续 20min）还是应用 mGluR 激动剂 DHPG（40μM，超灌注 10min），都能成功诱导至少 1 小时的双侧 LTD。此外，在前连合消融后，对侧 fEPSP 和 LTP 均消失。总之，本研究结果表明，斑马鱼端脑 Dl 与对侧 Dm 之间的投射是通过前连合进行的，并且具有突触可塑性。
