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对人类粪便样本进行鸟枪法宏基因组数据检测,以表征根除治疗后肠道微生物谱的变化。

Shotgun metagenomic data on the human stool samples to characterize shifts of the gut microbial profile after the eradication therapy.

作者信息

Boulygina Eugenia A, Markelova Maria I, Khusnutdinova Dilyara R, Siniagina Maria N, Malanin Sergey Yu, Abdulkhakov Rustam A, Abdulkhakov Sayar R, Chernov Vladislav M, Grigoryeva Tatiana V

机构信息

Kazan Federal University, 18 Kremlyovskaya, 420008 Russia.

Kazan State Medical University, 49 Butlerova, 420012 Russia.

出版信息

Data Brief. 2017 Jul 28;14:458-461. doi: 10.1016/j.dib.2017.07.070. eCollection 2017 Oct.

Abstract

The shotgun sequencing data presented in this report are related to the research article named "Gut microbiome shotgun sequencing in assessment of microbial community changes associated with eradication therapy" (Khusnutdinova et al., 2016) [1]. Typically, the eradication protocol includes a prolonged two-week use of the broad-spectrum antibiotics. The presented data on the whole-genome sequencing of the total DNA from stool samples of patients before the start of the eradication, immediately after eradication and several weeks after the end of treatment could help to profile the gut microbiota both taxonomically and functionally. The presented data together with those described in Glushchenko et al. (2017) [2] allow researchers to characterize the metagenomic profiles in which the use of antibiotics could result in dramatic changes in the intestinal microbiota composition. We perform 15 gut metagenomes from 5 patients with infection, obtained through the shotgun sequencing on the SOLiD 5500 W platform. Raw reads are deposited in the ENA under project ID PRJEB21338.

摘要

本报告中呈现的鸟枪法测序数据与名为《根除疗法相关微生物群落变化评估中的肠道微生物组鸟枪法测序》(Khusnutdinova等人,2016年)[1]的研究文章相关。通常,根除方案包括长期使用两周的广谱抗生素。所呈现的关于患者在根除治疗开始前、根除后即刻以及治疗结束后数周粪便样本中总DNA的全基因组测序数据,有助于从分类学和功能学角度描绘肠道微生物群。所呈现的数据与Glushchenko等人(2017年)[2]中描述的数据一起,使研究人员能够表征宏基因组图谱,其中抗生素的使用可能导致肠道微生物群组成发生显著变化。我们通过在SOLiD 5500 W平台上进行鸟枪法测序,从5名感染患者中获得了15个肠道宏基因组。原始读数存于ENA,项目编号为PRJEB21338。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/948f/5554923/d716ba81f28f/mmc1.jpg

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本文引用的文献

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