Uffo Odalys, Martínez Nadia, Acosta Atzel, Sanz Arianne, Martín-Burriel Inmaculada, Osta Rosario, Rodellar Clementina, Zaragoza Pilar
Laboratorio de Genética Molecular,Centro Nacional de Sanidad Agropecuaria,Apartado 10,PO 32700,San José de las Lajas,Mayabeque,Cuba.
LAGENBIO, Facultad de Veterinaria,IA2, Universidad de Zaragoza,Miguel Servet 177, 50013,Zaragoza,España.
J Dairy Res. 2017 Aug;84(3):289-292. doi: 10.1017/S0022029917000425.
The aim of this Regional Research Communication was to validate a panel of 30 microsatellite markers recommended by FAO/ISAG for studies of biodiversity in cattle to improve the characterisation of Cuban buffalo populations. The water buffalo (Bubalus bubalis) is an economically important livestock species. Therefore, research focused on the study of the genetic relationships among water buffalo populations is useful to support conservation decisions and to design breeding schemes. Twenty-eight of the 30 tested regions were amplified, one of which (ETH10) turned out to be monomorphic. A total of 143 alleles were observed in the Cuban water buffalo population. The average number of alleles per locus was 5·04. The number of alleles per polymorphic locus ranged from two (INRA 63 and MM12) to nine (ETH185). The observed and expected heterozygosity ranged from 0·108 (HAUT24) to 0·851 (CSSM66) and 0·104 (MM12) to 0·829(INRA32), respectively. The polymorphic information content (PIC) ranged from 0·097 (MM12) to 0·806 (INRA32), and the overall value for these markers was 0·482. Within the population, inbreeding estimates (F IS) was positive in 14 of the 30 loci analysed. This study thus highlights the usefulness of heterologous bovine microsatellite markers to assess the genetic variability in Cuban water buffalo breeds. Furthermore, the results can be utilised for future breeding strategies and conservation.
本次区域研究通讯的目的是验证粮农组织/国际动物遗传学会推荐的一组30个微卫星标记，用于牛的生物多样性研究，以改进古巴水牛种群的特征描述。水牛（Bubalus bubalis）是一种具有重要经济意义的家畜物种。因此，专注于研究水牛种群之间遗传关系的研究有助于支持保护决策和设计育种方案。30个测试区域中有28个被扩增，其中一个（ETH10）结果显示为单态性。在古巴水牛种群中总共观察到143个等位基因。每个位点的平均等位基因数为5.04。每个多态性位点的等位基因数从两个（INRA 63和MM12）到九个（ETH185）不等。观察到的杂合度和预期杂合度分别从0.108（HAUT24）到0.851（CSSM66）以及从0.104（MM12）到0.829（INRA32）。多态信息含量（PIC）从0.097（MM12）到0.806（INRA32）不等，这些标记的总体值为0.482。在种群内部，在分析的30个位点中有14个的近亲繁殖估计值（F IS）为正。因此，本研究突出了异源牛微卫星标记在评估古巴水牛品种遗传变异性方面的有用性。此外，研究结果可用于未来的育种策略和保护工作。
